Soluble Sugar Content and Metabolism as Related to the Heat-Induced Chilling Tolerance of Loquat Fruit During Cold Storage

• Published in: Food and bioprocess technology 2013-12, Vol.6 (12), p.3490-3498
• Main Authors: Shao, Xingfeng, Zhu, Yong, Cao, Shifeng, Wang, Hongfei, Song, Yuxing
• Format: Article
• Language: English
• Subjects: Agriculture; Air temperature; Ascorbic acid; Biotechnology; Brittleness; Browning; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Chilling; Cold storage; Cold tolerance; Cooling; Fatty acids; Food Science; Fructose; Fruits; Glucose; Glutathione; Glutathione reductase; Heat treatment; Heat treatments; High temperature; Hydrogen peroxide; Invertase; L-Ascorbate peroxidase; Malondialdehyde; Membrane permeability; Metabolism; Original Paper; Peroxidase; Reductases; Sucrose; Sucrose phosphate synthase; Sucrose synthase; Sugar
• ISSN: 1935-5130; 1935-5149
• DOI: 10.1007/s11947-012-1011-6

The effects of high-temperature, short-time hot air treatment (45 °C for 3 h) on soluble sugar metabolism and chilling tolerance in loquat fruit stored at 5 °C for 5 weeks were investigated. Heat treatment significantly reduced chilling severity, as evidenced by lower firmness and internal browning and higher levels of extractable juice. Meanwhile, this treatment accelerated the activities of acid invertase, neutral invertase, sucrose phosphate synthase and sucrose synthase during storage. However, sucrose degradation was predominant, which caused lower levels of sucrose and higher levels of glucose and fructose in the heat-treated group. In addition, the ascorbate acid content and the activities of ascorbate peroxidase and glutathione reductase in the heat-treated fruit were much higher than those in control fruit, resulting in lower levels of hydrogen peroxide (H 2 O 2 ) and malondialdehyde and decreased membrane permeability as well as a higher unsaturated/saturated fatty acid ratio at the end of storage. Our results suggest that the increased levels of reducing sugars, especially those of glucose, may induce the ascorbate–glutathione cycle activity to scavenge for H 2 O 2 , whose content relates to the heat-induced chilling tolerance of loquat fruit.