A simple, economical, and high efficient protocol to produce "in vitro" miniature rose

Due to its high commercial value, many studies on rose (Rosa hybrida L.) micropropagation have been published. However, there are a limited number of studies on rose in vitro flowering. These studies only focused on the roles of plant growth regulators in the formation and morphogenesis of flowers....

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 2020-06, Vol.56 (3), p.362-365
Main Authors: Nguyen, Nguyen Hoai, Van Le, Bui
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Cell Biology
Cultivars
Cytokinins
Developmental Biology
Flowering
Flowers
Flowers & plants
Growth regulators
Life Sciences
Micropropagation
Morphogenesis
Plant Breeding/Biotechnology
Plant Genetics and Genomics
Plant growth
Plant Sciences
PLANT TISSUE CULTURE
Plants (botany)
Recycling centers
Shoots
Studies
Subculture
Sucrose
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Summary:Due to its high commercial value, many studies on rose (Rosa hybrida L.) micropropagation have been published. However, there are a limited number of studies on rose in vitro flowering. These studies only focused on the roles of plant growth regulators in the formation and morphogenesis of flowers. In this protocol, cytokinin was confirmed to positively function in the induction of m vitro rose flowers. In fact, more than 40% of in vitro shoots were induced to flower when they were grown on a medium supplemented with benzylaminopurine (BA) (2 mg L⁻¹) and IAA (0.1 mg L⁻¹). In addition, this study showed that the growth medium supplemented with only coconut water (15 or 20% v/v) was very efficient to induce flowering oí in vitro miniature rose plants (> 70%) after 60 d of subculture. In addition, the in vitro flowers were normal and almost similar to ex vitro flowers in terms of flower shape and color. Based on these results, a detailed procedure for in vitro miniature rose flower production is provided.
ISSN:1054-5476
1475-2689
DOI:10.1007/s11627-019-10043-1