2. DIVERSITY OF THE POPULATION OF SHIGELLA STRAINS CIRCULATING ON ROMANIAN TERRITORY ISOLATED BETWEEN 2016-2018

This aim of this study was the phenotypic and genotypic characterization of the virulence and antimicrobial resistance profile, as well as the genetic relatedness of the Shigella spp., strains collected during 2016-2018, with the purpose of enriching information on virulence potential, antimicrobial...

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Published in:Romanian archives of microbiology and immunology 2019-10, Vol.78 (4), p.213-214
Main Authors: Cristea, Daniela, Ciontea, Adriana Simona, Popa, Mircea Ioan, Oprea, Mihaela, Dinu, Sorin, Popa, Andrei, Andrei, Melania Mihaela, Zamfir, Mădălina, Condei, Maria, Zota, Lavinia, Nica, Maria, Usein, Codruţa Romaniţa
Format: Article
Language:English
Subjects:
Ampicillin
Antibiotic resistance
Antibiotics
Antimicrobial agents
Antimicrobial resistance
Cephalosporins
Ciprofloxacin
Drug resistance
Gel electrophoresis
Genotypes
Nalidixic acid
Public health
Shigella
Shigellosis
Strains (organisms)
Sulfamethoxazole
Trimethoprim
Virulence
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Summary:This aim of this study was the phenotypic and genotypic characterization of the virulence and antimicrobial resistance profile, as well as the genetic relatedness of the Shigella spp., strains collected during 2016-2018, with the purpose of enriching information on virulence potential, antimicrobial susceptibility and the genetic kinship. Materials and methods: A total of 86 strains of Shigella spp. were tested phenotypically to establish antibiotic resistance profiles. The analysis of the molecular basis of antibiotic resistance to extended spectrum cephalosporins was performed using a protocol based on the amplification of the ctxM gene, followed by the amplicon sequencing. The ipaH, ipaBCD, ial, sen, set1A, set1B, sat and pic virulence genes were detected by PCR technique. The genetic kinship was established using pulsed field gel electrophoresis (PFGE). Results: Overall, the resistance most commonly acquired by the autochthonous Shigella strains investigated was to the combination of trimethoprim with sulfamethoxazole (44/86 strains), and ampicillin (41/86 strains). Strains with resistance to extended spectrum cephalosporins (12/86 strains) were detected, as well as strains that displayed resistance to nalidixic acid (3/86 strains) but not to ciprofloxacin. The genes blaCTXM15 (2 strains), blaCTXM 3 (2 strains), blaCTXM 1 (8 strains) were identified. The virulence markers targeted ranged in prevalence from 6% (set1A, set1B, pic) to 100% (ipaH). The most complex virulence genotype was found in S. flexneri serotype 2a strains (ipaH, ipaBCD, ial, sen, sat, set1A, set1B, and pic genes). Molecular typing of Shigella spp. strains allowed the detection of several clusters with a similarity of at least 85%. Eight such clusters were identified among the S. sonnei strains and four clusters among the S. flexneri strains. Conclusions: Continued laboratory-based surveillance is essential to generate data that help to draw the picture of the public health problem represented by shigellosis in Romania.
ISSN:1222-3891
2601-9418