Determination of Cyclohexylmethylfluorophosphonate Metabolites in Human Blood Plasma Using High-Resolution Liquid Chromatography–Mass Spectrometry

Conditions for the extraction of a phosphonylated nonapeptide, phosphonylated tyrosine, and cyclohexylmethylphosphonic acid from human blood plasma followed by HPLC determination with detection by high-resolution tandem mass spectrometry were optimized. Conditions for human blood plasma sample prepa...

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Bibliographic Details
Published in:Journal of analytical chemistry (New York, N.Y.) N.Y.), 2020-06, Vol.75 (6), p.783-791
Main Authors: Braun, A. V., Stavitskaya, Ya. V., Baigil’diev, T. M., Oreshkin, D. V., Rybal’chenko, I. V., Rodin, I. A.
Format: Article
Language:English
Subjects:
Analytical Chemistry
Blood plasma
Chemistry
Chemistry and Materials Science
Elution
High performance liquid chromatography
High resolution
Ions
Markers
Mass spectrometry
Metabolites
Plasma
Scientific imaging
Spectroscopy
Tyrosine
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Summary:Conditions for the extraction of a phosphonylated nonapeptide, phosphonylated tyrosine, and cyclohexylmethylphosphonic acid from human blood plasma followed by HPLC determination with detection by high-resolution tandem mass spectrometry were optimized. Conditions for human blood plasma sample preparation, mass-spectrometric detection in selected reaction monitoring mode, and gradient elution were chosen to determine the above markers of exposure to cyclohexylmethylfluorophosphonate. The detection limit of the initial substance introduced into blood plasma was 0.05–0.1 ng/mL depending on the test marker.
ISSN:1061-9348
1608-3199
DOI:10.1134/S1061934820060027