High Performance Liquid Chromatography–Diode Array Detector Method for Benznidazole Quantitation in Lipid Based and Self Assembling Cyclodextrins Drug Delivery Systems

The challenge of the present work concerned in benznidazole assay in two drug delivery systems ( DDS ) which are different in the solubility character and sample complexity using HPLC−diode array detector ( DAD ) method. The lipid based DDS were mainly composed of medium-chain triglycerides and a mi...

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Bibliographic Details
Published in:Journal of analytical chemistry (New York, N.Y.) N.Y.), 2020-07, Vol.75 (7), p.922-929
Main Authors: Streck, Letícia, Doro Polyanne N de M, Fernandes-Pedrosa, Matheus F, da Silva-Júnior Arnóbio A
Format: Article
Language:English
Subjects:
Analysis
Analytical Chemistry
Arrays
Chemistry
Chemistry and Materials Science
Complex systems
Complexity
Control stability
Cyclodextrins
Data analysis
Detectors
Dextrins
Drug delivery systems
Drugs
High performance liquid chromatography
Linearity
Lipids
Methods
Quality control
Surface active agents
Triglycerides
Vehicles
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Summary:The challenge of the present work concerned in benznidazole assay in two drug delivery systems ( DDS ) which are different in the solubility character and sample complexity using HPLC−diode array detector ( DAD ) method. The lipid based DDS were mainly composed of medium-chain triglycerides and a mixture of surfactants, while the multicomponent inclusion complex systems involved cosolvents and different cyclodextrins. The method was validated according to the International Conference on Harmonization guidelines. The linearity of the standard curves was greater than 0.999 ( r 2 ) with the limits of detection of 0.05 µg/mL and quantification of 0.18 µg/mL. The method was specific, precise (RSD < 1.99%) and robust. The different drug interactions with systems did not alter the recovery which ranged from 93 to 106% for the distinct DDS. Statistical data analysis of different DDS demonstrated that selected parameters for HPLC−DAD method offered equivalent results and could be applied for stability studies and quality control of these drug delivery systems.
ISSN:1061-9348
1608-3199
DOI:10.1134/S1061934820070163