Identification and field testing of floral odorants that attract the rove beetle Pelecomalium testaceum (Mannerheim) to skunk cabbage, Lysichiton americanus (L.)

• Published in: Arthropod-plant interactions 2018-08, Vol.12 (4), p.591-599
• Main Authors: Brodie, Bekka S., Renyard, Asim, Gries, Regine, Zhai, Huimin, Ogilvie, Steven, Avery, Jennifer, Gries, Gerhard
• Format: Article
• Language: English
• Subjects: Animal behavior; Antennae; Aroma compounds; Beetles; Behavioral Sciences; Biomedical and Life Sciences; Carrion; Coleoptera; Ecology; Electroantennograms; Entomology; Feces; Field tests; Flowers; Gas chromatography; Headspace; Hydrocarbons; Indoles; Insects; Invertebrates; Life Sciences; Lysichiton americanus; Mass spectrometry; Mass spectroscopy; Odorants; Original Paper; Plant breeding; Plant Pathology; Plant Sciences; Pollen; Protomacronema testaceum; Redundancy; Semiochemicals; Symplocarpus; Traps
• ISSN: 1872-8855; 1872-8847
• DOI: 10.1007/s11829-018-9607-z

Western skunk cabbage, Lysichiton americanus (Araceae), is pollinated mainly by the rove beetle Pelecomalium testaceum (Staphylinidae). Our objective was to determine the floral semiochemical(s) of L. americanus that attract(s) P. testaceum . Porapak Q headspace volatile extracts of L. americanus inflorescences were analyzed by gas chromatographic–electroantennographic detection (GC–EAD) and GC–mass spectrometry. In GC–EAD analyses, three floral odorants [( E )-4 nonene, ( E )-5-undecene, indole] elicited consistent responses from the antennae of female P. testaceum . In field experiments, traps baited with a blend of these three components (“3-CB”) captured significantly more P. testaceum than unbaited control traps. Traps baited with the 3-CB, the two hydrocarbons, or indole, each captured significantly more beetles than unbaited control traps, indicating redundancy in the semiochemical blend. Moreover, traps baited with indole captured significantly more beetles than traps baited with either the 3-CB, or the hydrocarbons, indicating that indole is a key floral attractant for P. testaceum . Many necrophilous and coprophilous insects respond to indole in search of carrion or feces, but P. testaceum has never been associated with these types of resources. Both electrophysiological and behavioral responses of P. testaceum to two hydrocarbon semiochemicals, which are not signature odorants of carrion or feces, may indicate that the beetles recognize the odor of L. americanus as an honest signal, seek and pollinate its inflorescences, and get rewarded with pollen and on-plant mating opportunities.