Arsenic speciation in the bracket fungus Fomitopsis betulina from contaminated and pristine sites

• Published in: Environmental geochemistry and health 2020-09, Vol.42 (9), p.2723-2732
• Main Authors: Button, Mark, Koch, Iris, Watts, Michael J., Reimer, Kenneth J.
• Format: Article
• Language: English
• Subjects: Anions; Arsenates; Arsenic; Arsenite; Cations; Distribution; Earth and Environmental Science; Environment; Environmental Chemistry; Environmental Health; Fruit bodies; Fungi; Geochemistry; High performance liquid chromatography; HPLC; Inductively coupled plasma mass spectrometry; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Mushrooms; Original Paper; Oxygen; Pores; Porosity; Public Health; Soil; Soil contamination; Soil Science & Conservation; Speciation; Species; Sulfur; Sulphur; Terrestrial Pollution; Trace levels; Uptake; Woodlands; X ray absorption
• ISSN: 0269-4042; 1573-2983
• DOI: 10.1007/s10653-019-00506-0

Uptake, distribution and speciation of arsenic (As) were determined in the bracket fungus Fomitopsis betulina (previously Piptoporus betulinus ), commonly known as the birch polypore, collected from a woodland adjacent to a highly contaminated former mine in the Southwest UK and at an uncontaminated site in Quebec, Canada, with no past or present mining activity. The fruiting body was divided into cap, centre and pores representing the top, middle and underside to identify trends in the distribution and transformation of As. Total As, determined by inductively coupled plasma–mass spectrometry (ICP–MS), was approximately tenfold higher in the mushroom from the contaminated compared to the uncontaminated site. Overall, accumulation of As was low relative to values reported for some soil-dwelling species, with maximum levels of 1.6 mg/kg at the contaminated site. Arsenic speciation was performed on aqueous extracts via both anion and cation high-performance liquid chromatography–ICP–MS (HPLC–ICP–MS) and on whole dried samples using X-ray absorption near edge structure (XANES) analysis. Seven As species were detected in F. betulina from the contaminated site by HPLC–ICP–MS: arsenite (As III ), arsenate (As V ), dimethylarsinate (DMA V ), methylarsonate (MA V ), trimethylarsine oxide (TMAO), tetramethylarsonium ion (Tetra) and trace levels of arsenobetaine (AB). The same As species were observed at the uncontaminated site with the exception of TMAO and Tetra. Arsenic species were localized throughout the fruiting body at the contaminated site, with the cap and pores containing a majority of As V , only the cap containing TMAO, and the pores containing higher concentrations of DMA V and MA V as well as tetra and a trace of AB. XANES analysis demonstrated that the predominant form of As at the contaminated site was inorganic As III coordinated with sulphur or oxygen and As V coordinated with oxygen. This is the first account of arsenic speciation in F. betulina or any fungi of the family Fomitopsidaceae .