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Characterisation of the Pseudomonas savastanoi pv. phaseolicola population found in Eastern Australia associated with halo blight disease in Vigna radiata
This study analysed the phenotypic and genotypic variation among 511 Pseudomonas savastanoi pv. phaseolicola (Psp) isolates, causing halo blight in mungbeans. Collected from symptomatic mungbean ( Vigna radiata ) crops throughout Australia between 2005 and 2018, a total of 352 Psp isolates were phen...
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| Published in: | Australasian plant pathology 2020-09, Vol.49 (5), p.515-524 |
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| container_title | Australasian plant pathology |
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| creator | Noble, Thomas J. Young, Anthony J. Kelly, Lisa A. Barrerro, Roberto A. Douglas, Colin A. Long, Hao Williams, Brett Mundree, Sagadevan |
| description | This study analysed the phenotypic and genotypic variation among 511
Pseudomonas savastanoi
pv.
phaseolicola
(Psp) isolates, causing halo blight in mungbeans. Collected from symptomatic mungbean (
Vigna radiata
) crops throughout Australia between 2005 and 2018, a total of 352 Psp isolates were phenotypically screened. Our
in planta
screening against a set of four mungbean cultivars with known susceptible and resistant reactions revealed five distinctive pathotypes. Isolates belonging to pathotype 2 were the most prevalent at 84% and were found to be highly pathogenic towards all tested mungbean genotypes. Genomic variation was investigated for 205 isolates using DNA fingerprints, splitting the halo blight pathogen population into two broad genetic lineages. Further genetic testing for two known avirulence genes,
avrPphE
and
avrPphF
, identified the
avrPphE
gene in all the tested isolates and
avrPphF
present in all but two. To identify candidate avirulence genes unique to Psp isolates infecting mungbean in Australia, a comparative genomics study was undertaken on the whole-genome sequences of two epidemiologically important Psp isolates, T11544 and K4287. The information presented in this study has the potential to dramatically improve mungbean disease resistance now and into the future. |
| doi_str_mv | 10.1007/s13313-020-00722-8 |
| format | article |
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Pseudomonas savastanoi
pv.
phaseolicola
(Psp) isolates, causing halo blight in mungbeans. Collected from symptomatic mungbean (
Vigna radiata
) crops throughout Australia between 2005 and 2018, a total of 352 Psp isolates were phenotypically screened. Our
in planta
screening against a set of four mungbean cultivars with known susceptible and resistant reactions revealed five distinctive pathotypes. Isolates belonging to pathotype 2 were the most prevalent at 84% and were found to be highly pathogenic towards all tested mungbean genotypes. Genomic variation was investigated for 205 isolates using DNA fingerprints, splitting the halo blight pathogen population into two broad genetic lineages. Further genetic testing for two known avirulence genes,
avrPphE
and
avrPphF
, identified the
avrPphE
gene in all the tested isolates and
avrPphF
present in all but two. To identify candidate avirulence genes unique to Psp isolates infecting mungbean in Australia, a comparative genomics study was undertaken on the whole-genome sequences of two epidemiologically important Psp isolates, T11544 and K4287. The information presented in this study has the potential to dramatically improve mungbean disease resistance now and into the future.</description><identifier>ISSN: 0815-3191</identifier><identifier>EISSN: 1448-6032</identifier><identifier>DOI: 10.1007/s13313-020-00722-8</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Agriculture ; Beans ; Biomedical and Life Sciences ; Blight ; Cultivars ; Deoxyribonucleic acid ; Disease resistance ; DNA ; Ecology ; Entomology ; Epidemiology ; Gene sequencing ; Genes ; Genetic screening ; Genotypes ; Halo blight ; Life Sciences ; Original Paper ; Phenotypic variations ; Plant Pathology ; Plant Sciences ; Population genetics ; Pseudomonas ; Pseudomonas savastanoi ; Vigna radiata</subject><ispartof>Australasian plant pathology, 2020-09, Vol.49 (5), p.515-524</ispartof><rights>The Author(s) 2020</rights><rights>The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c363t-9b90576f0540c3cc6e69752f4d28f1c3daed97418d71637b4b4e4ca490b432823</citedby><cites>FETCH-LOGICAL-c363t-9b90576f0540c3cc6e69752f4d28f1c3daed97418d71637b4b4e4ca490b432823</cites><orcidid>0000-0002-7731-5559</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids></links><search><creatorcontrib>Noble, Thomas J.</creatorcontrib><creatorcontrib>Young, Anthony J.</creatorcontrib><creatorcontrib>Kelly, Lisa A.</creatorcontrib><creatorcontrib>Barrerro, Roberto A.</creatorcontrib><creatorcontrib>Douglas, Colin A.</creatorcontrib><creatorcontrib>Long, Hao</creatorcontrib><creatorcontrib>Williams, Brett</creatorcontrib><creatorcontrib>Mundree, Sagadevan</creatorcontrib><title>Characterisation of the Pseudomonas savastanoi pv. phaseolicola population found in Eastern Australia associated with halo blight disease in Vigna radiata</title><title>Australasian plant pathology</title><addtitle>Australasian Plant Pathol</addtitle><description>This study analysed the phenotypic and genotypic variation among 511
Pseudomonas savastanoi
pv.
phaseolicola
(Psp) isolates, causing halo blight in mungbeans. Collected from symptomatic mungbean (
Vigna radiata
) crops throughout Australia between 2005 and 2018, a total of 352 Psp isolates were phenotypically screened. Our
in planta
screening against a set of four mungbean cultivars with known susceptible and resistant reactions revealed five distinctive pathotypes. Isolates belonging to pathotype 2 were the most prevalent at 84% and were found to be highly pathogenic towards all tested mungbean genotypes. Genomic variation was investigated for 205 isolates using DNA fingerprints, splitting the halo blight pathogen population into two broad genetic lineages. Further genetic testing for two known avirulence genes,
avrPphE
and
avrPphF
, identified the
avrPphE
gene in all the tested isolates and
avrPphF
present in all but two. To identify candidate avirulence genes unique to Psp isolates infecting mungbean in Australia, a comparative genomics study was undertaken on the whole-genome sequences of two epidemiologically important Psp isolates, T11544 and K4287. The information presented in this study has the potential to dramatically improve mungbean disease resistance now and into the future.</description><subject>Agriculture</subject><subject>Beans</subject><subject>Biomedical and Life Sciences</subject><subject>Blight</subject><subject>Cultivars</subject><subject>Deoxyribonucleic acid</subject><subject>Disease resistance</subject><subject>DNA</subject><subject>Ecology</subject><subject>Entomology</subject><subject>Epidemiology</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genetic screening</subject><subject>Genotypes</subject><subject>Halo blight</subject><subject>Life Sciences</subject><subject>Original Paper</subject><subject>Phenotypic variations</subject><subject>Plant Pathology</subject><subject>Plant Sciences</subject><subject>Population genetics</subject><subject>Pseudomonas</subject><subject>Pseudomonas savastanoi</subject><subject>Vigna radiata</subject><issn>0815-3191</issn><issn>1448-6032</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kc1q3DAURkVJoZNpX6ArQddO9ee_ZRgmaWEgWSTdimtJHmtwJEdXTumr9GnrqQPZZSUunPNpcQj5ytkVZ6z-jlxKLgsmWLGcQhTNB7LhSjVFxaS4IBvW8LKQvOWfyCXiiTGuKsk25O9ugAQmu-QRso-Bxp7mwdF7dLONTzEAUoQXwAwhejq9XNFpAHRx9CaOQKc4zeNq9nEOlvpA9wvtUqDXM-YEowcKiNF4yM7S3z4PdIAx0m70xyFT69Etg2fxlz8GoAnsgsJn8rGHEd2X13dLHm_2D7sfxeHu9ufu-lAYWclctF3LyrrqWamYkcZUrmrrUvTKiqbnRlpwtq0Vb2zNK1l3qlNOGVAt65QUjZBb8m3dnVJ8nh1mfYpzCsuXWiipGtkqfqbESpkUEZPr9ZT8E6Q_mjN9bqDXBnppoP830M0iyVXCBQ5Hl96m37H-AbZzjHc</recordid><startdate>20200901</startdate><enddate>20200901</enddate><creator>Noble, Thomas J.</creator><creator>Young, Anthony J.</creator><creator>Kelly, Lisa A.</creator><creator>Barrerro, Roberto A.</creator><creator>Douglas, Colin A.</creator><creator>Long, Hao</creator><creator>Williams, Brett</creator><creator>Mundree, Sagadevan</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0002-7731-5559</orcidid></search><sort><creationdate>20200901</creationdate><title>Characterisation of the Pseudomonas savastanoi pv. phaseolicola population found in Eastern Australia associated with halo blight disease in Vigna radiata</title><author>Noble, Thomas J. ; Young, Anthony J. ; Kelly, Lisa A. ; Barrerro, Roberto A. ; Douglas, Colin A. ; Long, Hao ; Williams, Brett ; Mundree, Sagadevan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-9b90576f0540c3cc6e69752f4d28f1c3daed97418d71637b4b4e4ca490b432823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Agriculture</topic><topic>Beans</topic><topic>Biomedical and Life Sciences</topic><topic>Blight</topic><topic>Cultivars</topic><topic>Deoxyribonucleic acid</topic><topic>Disease resistance</topic><topic>DNA</topic><topic>Ecology</topic><topic>Entomology</topic><topic>Epidemiology</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genetic screening</topic><topic>Genotypes</topic><topic>Halo blight</topic><topic>Life Sciences</topic><topic>Original Paper</topic><topic>Phenotypic variations</topic><topic>Plant Pathology</topic><topic>Plant Sciences</topic><topic>Population genetics</topic><topic>Pseudomonas</topic><topic>Pseudomonas savastanoi</topic><topic>Vigna radiata</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Noble, Thomas J.</creatorcontrib><creatorcontrib>Young, Anthony J.</creatorcontrib><creatorcontrib>Kelly, Lisa A.</creatorcontrib><creatorcontrib>Barrerro, Roberto A.</creatorcontrib><creatorcontrib>Douglas, Colin A.</creatorcontrib><creatorcontrib>Long, Hao</creatorcontrib><creatorcontrib>Williams, Brett</creatorcontrib><creatorcontrib>Mundree, Sagadevan</creatorcontrib><collection>SpringerOpen</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Australasian plant pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Noble, Thomas J.</au><au>Young, Anthony J.</au><au>Kelly, Lisa A.</au><au>Barrerro, Roberto A.</au><au>Douglas, Colin A.</au><au>Long, Hao</au><au>Williams, Brett</au><au>Mundree, Sagadevan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterisation of the Pseudomonas savastanoi pv. phaseolicola population found in Eastern Australia associated with halo blight disease in Vigna radiata</atitle><jtitle>Australasian plant pathology</jtitle><stitle>Australasian Plant Pathol</stitle><date>2020-09-01</date><risdate>2020</risdate><volume>49</volume><issue>5</issue><spage>515</spage><epage>524</epage><pages>515-524</pages><issn>0815-3191</issn><eissn>1448-6032</eissn><abstract>This study analysed the phenotypic and genotypic variation among 511
Pseudomonas savastanoi
pv.
phaseolicola
(Psp) isolates, causing halo blight in mungbeans. Collected from symptomatic mungbean (
Vigna radiata
) crops throughout Australia between 2005 and 2018, a total of 352 Psp isolates were phenotypically screened. Our
in planta
screening against a set of four mungbean cultivars with known susceptible and resistant reactions revealed five distinctive pathotypes. Isolates belonging to pathotype 2 were the most prevalent at 84% and were found to be highly pathogenic towards all tested mungbean genotypes. Genomic variation was investigated for 205 isolates using DNA fingerprints, splitting the halo blight pathogen population into two broad genetic lineages. Further genetic testing for two known avirulence genes,
avrPphE
and
avrPphF
, identified the
avrPphE
gene in all the tested isolates and
avrPphF
present in all but two. To identify candidate avirulence genes unique to Psp isolates infecting mungbean in Australia, a comparative genomics study was undertaken on the whole-genome sequences of two epidemiologically important Psp isolates, T11544 and K4287. The information presented in this study has the potential to dramatically improve mungbean disease resistance now and into the future.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s13313-020-00722-8</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-7731-5559</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Australasian plant pathology, 2020-09, Vol.49 (5), p.515-524 |
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| language | eng |
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| source | Springer Link |
| subjects | Agriculture Beans Biomedical and Life Sciences Blight Cultivars Deoxyribonucleic acid Disease resistance DNA Ecology Entomology Epidemiology Gene sequencing Genes Genetic screening Genotypes Halo blight Life Sciences Original Paper Phenotypic variations Plant Pathology Plant Sciences Population genetics Pseudomonas Pseudomonas savastanoi Vigna radiata |
| title | Characterisation of the Pseudomonas savastanoi pv. phaseolicola population found in Eastern Australia associated with halo blight disease in Vigna radiata |
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