Engineering biosynthetic enzymes for industrial natural product synthesis (This manuscript has been authored in part by UT-Battelle, LLC, under contract DE-AC05-00OR22725 with the US Department of Energy (DOE). The US government retains and the publisher, by accepting the article for publication, acknowledges that the US government retains a nonexclusive, paid-up, irrevocable, worldwide license to publish or reproduce the published form of this manuscript, or allow others to do so, for US govern

Covering: 2000 to 2020Natural products and their derivatives are commercially important medicines, agrochemicals, flavors, fragrances, and food ingredients. Industrial strategies to produce these structurally complex molecules encompass varied combinations of chemical synthesis, biocatalysis, and ex...

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Bibliographic Details
Published in:Natural product reports 2020-01, Vol.37 (8), p.1122-1143
Main Authors: Galanie, Stephanie, Entwistle, David, Lalonde, James
Format: Article
Language:English
Subjects:
Agrochemicals
Algorithms
Biosynthesis
Catalysis
Chemical synthesis
Engineering
Enzymes
Federal agencies
Food industry
Fragrances
Learning algorithms
Machine learning
Microorganisms
Natural products
Public access
Strains (organisms)
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Summary:Covering: 2000 to 2020Natural products and their derivatives are commercially important medicines, agrochemicals, flavors, fragrances, and food ingredients. Industrial strategies to produce these structurally complex molecules encompass varied combinations of chemical synthesis, biocatalysis, and extraction from natural sources. Interest in engineering natural product biosynthesis began with the advent of genetic tools for pathway discovery. Genes and strains can now readily be synthesized, mutated, recombined, and sequenced. Enzyme engineering has succeeded commercially due to the development of genetic methods, analytical technologies, and machine learning algorithms. Today, engineered biosynthetic enzymes from organisms spanning the tree of life are used industrially to produce diverse molecules. These biocatalytic processes include single enzymatic steps, multienzyme cascades, and engineered native and heterologous microbial strains. This review will describe how biosynthetic enzymes have been engineered to enable commercial and near-commercial syntheses of natural products and their analogs.
ISSN:0265-0568
1460-4752
DOI:10.1039/c9np00071b