Cholecystokinin 1 and 2 in red seabream Pagrus major: molecular cloning, response to feeding, and a potential indicator of dietary protein source quality

Cholecystokinin (Cck) and peptide YY (Pyy) play important roles in digestive regulation in fish, but their function in red seabream ( Pagrus major ) remains obscure. Two cck complementary DNAs ( cck-1 and cck-2 ) in red seabream were identified, and tissue distribution analysis of the genes revealed...

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Bibliographic Details
Published in:Fisheries science 2020-09, Vol.86 (5), p.835-849
Main Authors: Huong, Tran Thi Mai, Murashita, Koji, Senzui, Ayaka, Matsumoto, Toshiro, Fukada, Haruhisa
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Body weight
Cholecystokinin
Cloning
Digestive system
Feeding
Fish
Fish & Wildlife Biology & Management
Fish meal
Fishmeal
Food Science
Freshwater & Marine Ecology
Gastrointestinal tract
Genes
Hindgut
Life Sciences
Marine fishes
Oral administration
Original Article
Pagrus major
Protein sources
Proteins
Pyloric caeca
Stomach
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Summary:Cholecystokinin (Cck) and peptide YY (Pyy) play important roles in digestive regulation in fish, but their function in red seabream ( Pagrus major ) remains obscure. Two cck complementary DNAs ( cck-1 and cck-2 ) in red seabream were identified, and tissue distribution analysis of the genes revealed that cck and pyy were expressed throughout the digestive tract, except for stomach cck-2 . After feeding, cck-1 expression significantly increased in the stomach and pyloric caeca, cck-2 expression significantly increased in the pyloric caeca and hindgut, and pyy expression increased in the pyloric caeca of red seabream. After oral administration of dietary protein, i.e., high-quality fish meal (HQFM), soy protein concentrate (SPC), or low-quality FM (LQFM), only HQFM significantly increased stomach cck-1 expression. To assess the growth of red seabream in response to different sources of dietary protein, three isonitrogenous and isoenergetic diets were formulated: HQFM, SPC with a low percentage of HQFM, and LQFM. Mean body weight was highest in the HQFM group after 6 weeks. The stomach cck-1 response to HQFM may explain the high growth in the HQFM group. Our data suggest that cck-1 , cck-2 , and pyy are involved in digestion. Furthermore, stomach cck-1 may be a valuable tool for evaluating the quality of dietary protein sources for red seabream.
ISSN:0919-9268
1444-2906
DOI:10.1007/s12562-020-01443-z