Atomic oxygen radical‐induced intracellular oxidization of mould spore cells

The inactivation mechanism of mould spores using plasma‐generated neutral reactive oxygen species (ROS) was investigated in this paper. Typical cell viability using a counting of colony‐forming unit assay indicated a major state of the spore cells. Both intracellular and extracellular damages have b...

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Bibliographic Details
Published in:Plasma processes and polymers 2020-10, Vol.17 (10), p.n/a
Main Authors: Tanaka, Yuta, Oh, Jun‐Seok, Hashizume, Hiroshi, Ohta, Takayuki, Kato, Masashi, Hori, Masaru, Ito, Masafumi
Format: Article
Language:English
Subjects:
atmospheric‐pressure plasma
Atomic oxygen
atomic oxygen radicals
Cell membranes
Deactivation
Electron microscopy
Emission microscopy
Fluorescence
Fungi
intracellular oxidization
Lipids
Microscopy
mould spore cells
Spores
Visualization
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Summary:The inactivation mechanism of mould spores using plasma‐generated neutral reactive oxygen species (ROS) was investigated in this paper. Typical cell viability using a counting of colony‐forming unit assay indicated a major state of the spore cells. Both intracellular and extracellular damages have been investigated by a couple of well‐established cell visualization techniques: confocal fluorescence emission microscopy, scanning electron microscopy, and transmission electron microscopy. It is revealed in this paper that the combination of the spore cell viability with those visualization results strongly suggested an early stage of the cell inactivation when ROS can induce intracellular lipid peroxidation through both nanometer‐thick lipid cell membrane and less‐damaged several hundred nanometer‐thick cell wall. The inactivation mechanism of mould spores using plasma‐generated neutral reactive oxygen species (ROS) was investigated in this paper. Typical cell viability using a counting of colony‐forming unit assay indicated a major state of the spore cells. Both intracellular and extracellular damages have been investigated by a couple of well‐established cell visualization techniques: a confocal fluorescence emission microscopy, scanning electron microscopy, and transmission electron microscopy. It is revealed in this paper that the combination of the spore cell viability with those visualization results strongly suggested an early stage of the cell inactivation when ROS can induce intracellular lipid peroxidation through both nanometer‐thick lipid cell membrane and less‐damaged several hundred nanometer‐thick cell wall.
ISSN:1612-8850
1612-8869
DOI:10.1002/ppap.202000001