UHPLC–MS/MS method for iohexol determination in human EDTA and lithium-heparin plasma, human urine and in goat- and pig EDTA plasma

Iohexol plasma clearance is used as an indicator of kidney function in clinical and preclinical settings. To investigate the pharmacokinetic profile of iohexol, a rapid, simple method for measurement of iohexol in different matrices and species was needed. Iohexol was separated on an Accucore C18 co...

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Bibliographic Details
Published in:Bioanalysis 2020-07, Vol.12 (14), p.981-990
Main Authors: Stevens, Jasper, Wessels, Mireille A, Roggeveld, Jan, Koster, Remco A, Dekkers, Claire C J, van Gelder, Maaike K, Gansevoort, Ron T, Heerspink, Hiddo J L, Touw, Daan J
Format: Article
Language:English
Subjects:
Accuracy
Acids
Anticoagulants
Calibration
Clinical medicine
Edetic acid
Experiments
goat plasma
Heparin
human plasma
human urine
iohexol
Kidney diseases
Laboratory animals
LC–MS/MS
Lithium
Methods
Pharmacokinetics
pig plasma
Plasma
Urine
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Summary:Iohexol plasma clearance is used as an indicator of kidney function in clinical and preclinical settings. To investigate the pharmacokinetic profile of iohexol, a rapid, simple method for measurement of iohexol in different matrices and species was needed. Iohexol was separated on an Accucore C18 column (Thermo Fisher Scientific, CA, USA). Detection was performed on a Thermo Scientific Quantiva tandem quadrupole mass spectrometer. The method was validated according to the requirements for bioanalytical methods issued by the US FDA and European Medicines Agency. We developed and validated a fast and efficient analytical method, suitable for analyzing iohexol in human EDTA plasma, human lithium-heparin plasma, human urine and goat- and pig EDTA plasma, using only one calibration line prepared in human EDTA plasma.
ISSN:1757-6180
1757-6199
DOI:10.4155/bio-2020-0122