Oral microbiota alteration associated with oral cancer and areca chewing

Oral cancer is among most common neoplasm of oral cavity; in many cases, it develops at the site of premalignant lesion. Areca nut has been identified as a carcinogen, which was proved to promote the inflammation level and contributes to oral malignancy. Chewing areca nut is the main cause of the pr...

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Bibliographic Details
Published in:Oral diseases 2021-03, Vol.27 (2), p.226-239
Main Authors: Zhong, Xiaohuan, Lu, Qin, Zhang, Qi, He, Yuan, Wei, Wenjuan, Wang, Yimin
Format: Article
Language:English
Subjects:
16S rRNA sequencing
area nut
Areca - adverse effects
Areca catechu
Buccal mucosa
carcinogenesis
Carcinogens
Chewing
Developmental stages
Fibrosis
Humans
Inflammation
Malignancy
Mastication
Microbiota
Microorganisms
Mouth Neoplasms - etiology
Nuts
Oral cancer
Oral carcinoma
Oral cavity
oral microbiota
Oral squamous cell carcinoma
oral submucous fibrosis
Oral Submucous Fibrosis - etiology
OSCC
Patients
Premalignant disease
RNA, Ribosomal, 16S - genetics
rRNA 16S
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Summary:Oral cancer is among most common neoplasm of oral cavity; in many cases, it develops at the site of premalignant lesion. Areca nut has been identified as a carcinogen, which was proved to promote the inflammation level and contributes to oral malignancy. Chewing areca nut is the main cause of the premalignant disease oral submucous fibrosis (OSF). Bacterial alterations were suggested to be assonated with oral cancer progression. Therefore, the present study was carried out to determine the changes of microbiota in the mucosa along stage of development of oral cancer with areca nut chewing. 162 participants, reporting to department of oral medical center, were enrolled into the study which includes 45 patients each of OSF, 42 of oral cancer, 29 healthy controls (HC) with areca nut chewing, and 46 healthy controls (HC) never chewing areca nut. Oral swabbing of tongue dorsum, buccal mucosa, and gingiva was evaluated by MiSeq platform of the V3–V4 region of the 16S rRNA gene. These data revealed microbial changes that may mirror oral cancer progression and reflect clinical preconditions such as areca nut chewing. Consequently, revealing microbial changes in patients with oral squamous cell carcinomas and the premalignant disease oral submucous fibrosis (OSF) with areca nut chewing might improve our understanding of the pathobiology of the disease and help in the design of novel diagnostic and treatment strategies.
ISSN:1354-523X
1601-0825
DOI:10.1111/odi.13545