Synthesis of biocompatible chitosan decorated silver nanoparticles biocomposites for enhanced antimicrobial and anticancer property

• Published in: Process biochemistry (1991) 2020-12, Vol.99, p.348-356
• Main Authors: Gopinath, V., MubarakAli, D., Vadivelu, Jamuna, Manjunath Kamath, S., Syed, Asad, Elgorban, Abdallah M.
• Format: Article
• Language: English
• Subjects: Antibacterial; Antibacterial activity; Anticancer properties; Apoptosis; Bactericidal activity; Biocompatibility; Biomedical materials; Biosynthesis; Capping; Chitosan; Composite materials; Cytotoxicity; Damage detection; DNA damage; Gram-negative bacteria; Lung cancer; Macrophages; Metabolic activity; Microwave radiation; Nanoparticles; Silver; Silver nanoparticles; Toxicity
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2020.09.011

[Display omitted] •Rapid, cost-effective PB-AgNPs synthesis and Chitosan capping was achieved.•UV–vis spectrum, SEM, and EDX analysis confirmed the nanoparticles synthesis.•CS@PB-AgNPs showed better bacterial inhibitory effect than uncapped PB-AgNPs.•CS capping demonstrated reduced cellular toxicity to RAW264.7 macrophages and toxic to A549 lung cancer cells. Numerous studies investigated the biosynthesis of silver nanoparticles (AgNPs); however, there is a large gap for the ideal time-consuming process and their cytotoxicity. Herein, for the first time, rapid AgNPs was synthesized in a short time span, using Piper betle leaf (PBL) extract by applying microwave exposure. PB-AgNPs antibacterial activity and cell compatibility were enhanced by capping with chitosan (CS@PB-AgNPs). The synthesized nanoparticles were characterized by bioanalytical techniques. PB-AgNPs expressed significant antibacterial activity against Gram-positive and Gram-negative bacterial pathogens, while hybrid CS@PB-AgNPs presented the enhanced bactericidal activity. In addition, PB-AgNPs exhibited IC50 value of 140 μg/mL against RAW 264.7 macrophages and 100 μg/mL against lung cancer cells while, CS capping reduced its toxicity at IC50 values of 400 μg/mL and 180 μg/mL respectively were affirmed by MTT, apoptosis and DNA damage detection. Overall it was demonstrated that CS capping could be a phenomenal finding to improve the biomedical potential of AgNPs.