Electrochemical sensor based on modified methylcellulose by graphene oxide and Fe3O4 nanoparticles: Application in the analysis of uric acid content in urine

• Published in: Journal of electroanalytical chemistry (Lausanne, Switzerland) Switzerland), 2020-11, Vol.877, p.114503, Article 114503
• Main Authors: Sohouli, Esmail, Khosrowshahi, Elnaz Marzi, Radi, Pantea, Naghian, Ebrahim, Rahimi-Nasrabadi, Mehdi, Ahmadi, Farhad
• Format: Article
• Language: English
• Subjects: Chemical sensors; Coated electrodes; Electrodes; Electron transfer; Glassy carbon; Glassy carbon electrode; Graphene; Graphene oxide; Hydrogels; Iron oxide nanoparticles; Iron oxides; Methylcellulose; Nanocomposites; Nanoparticles; Synergistic effect; Uric acid; Voltammetry
• ISSN: 1572-6657; 1873-2569
• DOI: 10.1016/j.jelechem.2020.114503

The modified glassy carbon electrode (GCE) with a coating containing methylcellulose/graphene oxide/iron oxide nano hydrogel (MC-GO-Fe3O4) has been developed to design a high-performance electrochemical bioassay system. The influence of each component of the modifier was investigated separately. This comparison showed that exist of all components in modification has a synergistic effect. MC-GO-Fe3O4 nanocomposite has a larger surface area and provides a more fine-grained media to facilitate electron transfer during the reaction between analyte and electrode. The modified GCE was used for the electrooxidation of uric acid (UA) as a model analyte by differential pulse voltammetry (DPV) and cyclic voltammetry (CV) techniques. It exhibits a diffusion-controlled process with a two-electron, two-proton transfer reaction. The ability of the developed electrode to measure UA was studied and it has been found a linear dependence on UA concentration with a linear amplitude of 0.5–140 μM and a limit of detection of 0.17 μM. According to the results, the GCE|MC-GO-Fe3O4 provides an accurate and precise assay pathway of UA in the urine sample. [Display omitted] •The nano hydrogel of MC-GO-Fe3O4 was synthesized by an ultrasonic-assisted method.•The MC-GO-Fe3O4 was used for the modification of a glassy carbon electrode.•The modified electrode was used as an electrochemical sensor for the determination of uric acid in the urine sample.•Characteristics of the developed sensor response in aqueous media and real sample matrix are capable.