Influence of ultraviolet and gamma ray irradiation on luminescent bacteria and exploring their efficacy as biosensors

Toxicity monitoring of harmful radiation is an indispensable issue in modern radioecology. As bioluminescent bacteria have the simplest structure to epitomize the biosphere, their bioluminescence can also act as an indicator of the conditions, therefore assay systems based on luminous bacteria can b...

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Bibliographic Details
Published in:Luminescence (Chichester, England) England), 2021-03, Vol.36 (2), p.525-530
Main Authors: Wanjari, Rashmi A, Shanware, Arti S, Dhoble, Sanjay J.
Format: Article
Language:English
Subjects:
Bacteria
Bioluminescence
biosensor
Biosensors
Biosphere
Comparative analysis
Comparative studies
DNA
Environmental monitoring
Exposure
Gamma irradiation
Gamma radiation
Gamma rays
Irradiation
Luminous bacteria
Microbiological strains
Phylogeny
Pollution monitoring
Polymerase chain reaction
Radiation
Radiation effects
Radioecology
Random amplified polymorphic DNA
RAPD
Sensors
Strains (organisms)
Toxicity
Ultraviolet radiation
UV irradiation
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Summary:Toxicity monitoring of harmful radiation is an indispensable issue in modern radioecology. As bioluminescent bacteria have the simplest structure to epitomize the biosphere, their bioluminescence can also act as an indicator of the conditions, therefore assay systems based on luminous bacteria can be used to monitor environmental radiotoxicity. The present investigation explored the measurement of bacterial luminescence, which can be easily computed. Bioluminescent bacterial strains were used to evaluate the effect of ultraviolet (UV) and gamma irradiation. A random amplified polymorphic DNA (RAPD) assay was carried out to observe alterations under exposure. Using a phylogenetic tree, a comparative study of the effect of UV and gamma rays was carried out. The isolated strains showed marked sensitivity towards radiation exposure present in the environment and therefore they could be used as potential biosensing elements for developing an on‐site pollution monitoring biosensor. Biobeads were constructed from a consortium of newly identified strains of Vibrio harveyi and Vibrio natriegens from Tarkarli beach and Baga beach, India. The biobeads showed marked sensitivity towards ultraviolet ray and gamma ray exposure, signifying their use as a potential biosensing agent for monitoring pollution.
ISSN:1522-7235
1522-7243
DOI:10.1002/bio.3972