Evaluation of alternative methodologies for dry matter degradability determination in the in vitro gas production method

• Published in: Journal of animal science 2020-11, Vol.98, p.430-430
• Main Authors: Lobo, Annelise G, Vincenzi, Rafaela, Lobo, Richard R, Benetel, Vanderlei, Panosso, Leticia L, Bermudez, Yuli Andrea, Bueno, Ives Claudio da silva
• Format: Article
• Language: English
• Subjects: Biodegradation; Crucibles; Degradability; Degradation; Design of experiments; Dry matter; Experimental design; Filters; Gas production; Gases; In vitro methods and tests; Oil and gas production; Production methods; Weighing
• ISSN: 0021-8812; 1525-3163

The use of filter crucibles to determine the degradability increases the time required in the analytical process, thus reducing the speed of obtaining experimental data. This has made it necessary to search for alternative methods that ensure a homogeneous digestion and filtration system for all samples and provide accurate and accurate results. The study evaluated the in vitro dry matter degradability of five treatments: 1) Filter crucible; 2) F57 bags (Ankom) with non-degradable mass; 3) TNT bags weighing g/m2 with non-degradable mass; 4) F57 bags (Ankom); 5) TNT bags weighing g/m2. The in vitro gas production method used was the semiautomatic one with pressure transducer, for that 8 bovines Nellore castrated male were used as content and ruminal liquid donors. A completely randomized experimental design was used, with 5 treatments, 4 blocks (inocula), and three replicates within each block. PROC GLM was used to adjust for multiple comparisons of the data using the Tukeys test at 5% significance. There was no difference in the in vitro dry matter degradability for 24 or 96 hours (P-value 0.0554 and 0.1411, respectively), regardless of the treatment. Thus, the possibility of using TNT made bag to the detriment of the F57 bag is affirmed because of the high cost of this one in relation to that one.