Ultrasensitive and selective molecularly imprinted electrochemical oxaliplatin sensor based on a novel nitrogen-doped carbon nanotubes/Ag@cu MOF as a signal enhancer and reporter nanohybrid

• Published in: Mikrochimica acta (1966) 2021-04, Vol.188 (4), p.124, Article 124
• Main Authors: Mahnashi, Mater H., Mahmoud, Ashraf M., Alhazzani, Khalid, Alanazi, A. Z., Alaseem, Ali Mohammed, Algahtani, Mohammad M., El-Wekil, Mohamed M.
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Antimitotic agents; Antineoplastic agents; Binding; Biosensors; Blood plasma; Carbon; Carbon nanotubes; Characterization and Evaluation of Materials; Chemical sensors; Chemistry; Chemistry and Materials Science; Copper; Copper - chemistry; Design optimization; Design standards; Electric properties; Electrochemical Techniques - instrumentation; Electrochemical Techniques - methods; Electrodes; Glassy carbon; Humans; Journalists; Limit of Detection; Metal Nanoparticles - chemistry; Metal-organic frameworks; Metal-Organic Frameworks - chemistry; Microengineering; Molecularly Imprinted Polymers - chemistry; Nanochemistry; Nanoparticles; Nanotechnology; Nanotubes; Nanotubes, Carbon - chemistry; Nitrogen; Nitrogen - chemistry; Original Paper; Oxaliplatin - analysis; Oxaliplatin - blood; Oxaliplatin - urine; Polymer films; Polymers; Polymers - chemistry; Pyrroles - chemistry; Reproducibility of Results; Selectivity; Sensors; Silver; Silver - chemistry
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-021-04781-6

A sensitive and selective molecular imprinted polymeric network (MIP) electrochemical sensor is proposed for the determination of anti-cancer drug oxaliplatin (OXAL). The polymeric network [poly(pyrrole)] was electrodeposited on a glassy carbon electrode (GCE) modified with silver nanoparticles (Ag) functionalized Cu-metal organic framework (Cu-BDC) and nitrogen-doped carbon nanotubes (N-CNTs). The MIP-Ag@Cu-BDC /N-CNTs/GCE showed an observable reduction peak at −0.14 V, which corresponds to the Cu-BDC reduction. This peak increased and decreased by eluting and rebinding of OXAL, respectively. The binding constant between OXAL and Cu-BDC was calculated to be 3.5 ± 0.1 × 10 7  mol −1  L. The electrochemical signal (∆i) increased with increasing OXAL concentration in the range 0.056–200 ng mL −1 with a limit of detection (LOD, S/ N  = 3) of 0.016 ng mL −1 . The combination of N-CNTs and Ag@Cu-BDC improves both the conductivity and the anchoring sites for binding the polymer film on the surface of the electrode. The MIP-based electrochemical sensor offered outstanding sensitivity, selectivity, reproducibility, and stability. The MIP-Ag@Cu-BDC /N-CNTs/GCE was applied to determine OXAL in pharmaceutical injections, human plasma, and urine samples with good recoveries (97.5–105%) and acceptable relative standard deviations (RSDs = 1.8–3.2%). Factors affecting fabrication of MIP and OXAL determination were optimized using standard orthogonal design using L 25 (5 6 ) matrix. This MIP based electrochemical sensor opens a new venue for the fabrication of other similar  sensors and biosensors. Graphical abstract