Molecular and phylogenetic analysis reveals new diversity of Dunaliella salina from hypersaline environments

Twelve hyper-β carotene-producing strains of algae assigned to the genus Dunaliella salina have been isolated from various hypersaline environments in Israel, South Africa, Namibia and Spain. Intron-sizing of the SSU rDNA and phylogenetic analysis of these isolates were undertaken using four commonl...

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Bibliographic Details
Published in:Journal of the Marine Biological Association of the United Kingdom 2021-02, Vol.101 (1), p.27-37
Main Authors: Highfield, Andrea, Ward, Angela, Pipe, Richard, Schroeder, Declan C.
Format: Article
Language:English
Subjects:
Algae
Carotene
DNA
Dunaliella salina
Economic analysis
Economic importance
Genes
Genetic analysis
Genetic diversity
Genetic variation
Genotyping
Morphology
Phylogenetics
Phylogeny
Strains (organisms)
Taxonomy
β-Carotene
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Summary:Twelve hyper-β carotene-producing strains of algae assigned to the genus Dunaliella salina have been isolated from various hypersaline environments in Israel, South Africa, Namibia and Spain. Intron-sizing of the SSU rDNA and phylogenetic analysis of these isolates were undertaken using four commonly employed markers for genotyping, LSU rDNA, ITS, rbcL and tufA and their application to the study of Dunaliella evaluated. Novel isolates have been identified and phylogenetic analyses have shown the need for clarification on the taxonomy of Dunaliella salina. We propose the division of D. salina into four sub-clades as defined by a robust phylogeny based on the concatenation of four genes. This study further demonstrates the considerable genetic diversity within D. salina and the potential of genetic analyses for aiding in the selection of prospective economically important strains.
ISSN:0025-3154
1469-7769
DOI:10.1017/S0025315420001319