Semi-enclosed microfluidic device on glass-fiber membrane with enhanced signal quality for colorimetric analyte detection in whole blood

Microfluidic analytical devices manufactured on paper and similar inexpensive substrates (µ-PADs) have shown considerable promise for disease diagnostics in resource-limited regions. However, current commercialization approaches can be improved substantially by addressing existing technical challeng...

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Bibliographic Details
Published in:Microfluidics and nanofluidics 2021-06, Vol.25 (6), Article 47
Main Authors: Raj, N., Breedveld, V., Hess, D. W.
Format: Article
Language:English
Subjects:
Albumin
Albumins
Analytical Chemistry
Biomedical Engineering and Bioengineering
Blood
Blood plasma
Cellulose
Colorimetry
Commercialization
Detection
Engineering
Engineering Fluid Dynamics
Fabrication
Glucose
Homogeneity
Marketing
Membranes
Microfluidic devices
Nanocrystals
Nanotechnology and Microengineering
Plasma
Research Paper
Separation
Signal quality
Substrates
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Summary:Microfluidic analytical devices manufactured on paper and similar inexpensive substrates (µ-PADs) have shown considerable promise for disease diagnostics in resource-limited regions. However, current commercialization approaches can be improved substantially by addressing existing technical challenges associated with µ-PADs. Among these, off-device plasma separation from whole blood is a critical challenge in µ-PAD technology that limits commercialization. Existing µ-PADs made by combining multiple components require extra fabrication steps and manufacturing material. Our approach utilizes a two-step plasma process to fabricate single-layer semi-enclosed µ-PADs directly on a commercially available blood plasma separation membrane to incorporate blood plasma separation functionality into the device. The semi-enclosed µ-PADs are bonded with low-cost adhesive plastic tape to provide mechanical support to the device and make it more mechanically robust for field applications. Detection zones of the µ-PADs have also been modified with a cellulose nanocrystal (CNC) to increase colorimetric signal homogeneity, thus enhancing signal quality. The CNC-modified µ-PADs have been used for colorimetric detection of two model analytes (glucose and albumin) in whole blood. Colorimetric signals for both glucose and albumin from whole blood samples were consistent with the calibration curves generated using stock solutions.
ISSN:1613-4982
1613-4990
DOI:10.1007/s10404-021-02447-6