Development and Validation of RP-HPLC Method for the Estimation of L-Methyl Folate and Tofisopam in combined Formulation

The non-methylated form, folic acid (vitamin B9), is a synthetic folate form and must undergo enzymatic reduction to become biologically active by methylenetetrahydrofolate reductase (MTHFR)1-3. Knowledge of its molecular weight, polarity, ionic character, pKa values, absorption wavelength, compound...

Full description

Saved in:
Bibliographic Details
Published in:Asian Journal of Research in Chemistry 2021, Vol.14 (1), p.1-9
Main Authors: Seelam, Mohan, Purimitla, Satyanarayana, Vedula, Nagalakshmi, Kandukuri, Sudheer Kumar, Gundumolu, Srinivasa Rao
Format: Article
Language:English
Subjects:
Accuracy
Acetonitrile
Acids
Binding sites
Chromatography
Filter paper
Flow velocity
Folic acid
High performance liquid chromatography
Ions
Liquid chromatography
Membrane filters
Pharmaceuticals
Polarity
Reductases
Sensors
Test methods
Vitamin B
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The non-methylated form, folic acid (vitamin B9), is a synthetic folate form and must undergo enzymatic reduction to become biologically active by methylenetetrahydrofolate reductase (MTHFR)1-3. Knowledge of its molecular weight, polarity, ionic character, pKa values, absorption wavelength, compound purity and solubility must be understood in order to create a new HPLC process for any drug.8,9 for the estimation of Lmethylfolate and Tofisopam in pharmaceutical dosage form, The objective of this research was to develop and validate a process of HPLC that was sensitive, accurate, precise and specific. Chromatographic Terms: Using Isocratic elution and Acetonitrile, chromatographic analysis was carried out: 0.1 percent PH modified to 2.5 with OPA (50:50 by volume) as a mobile phase and filtered through 0.45p membrane filter paper. Validating: The aim of the optimized chromatographic separation was to achieve a resolution above 1.5 between all components, the tailing factor is less than 2.0, and the plate count will be more than 2000 w.r. to the stationary, mobile phase compositions, and flow rate, volume of sample, wavelength and temperature detection.
ISSN:0974-4169
0974-4150
DOI:10.5958/0974-4150.2021.00005.5