Putative role of N-Arachidonoyl glycine (NAGly) Acute hyperphagia in BALB/c mice

Introduction: Obesity is a pandemic problem associated to development of chronic degenerative diseases. N-arachidonylglycine (NAGly) is speculated to participate in pain regulation, immunity and insulin secretion; some N-acyl amino acids induce hyperphagia; in light of this, NAGly metabolic function...

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Published in:Emirates journal of food and agriculture 2021-03, Vol.33 (3), p.245-252
Main Authors: Ernesto, Ramírez-Orozco Ricardo, Alejandra, Pedroza-García Karina, David, Masuoka, Alberto, Camacho-Morales, Anahí, Álvarez-Miramontes Silvia, Alejandro, Martínez-Miranda Christian
Format: Article
Language:English
Subjects:
Amino acids
Animal models
Body weight gain
Carbohydrates
Circuits
Cytokines
Diet
Energy balance
Enzyme-linked immunosorbent assay
Ethanol
Fatty acids
Food
Food intake
Food preferences
Glycine
High fat diet
Homeostasis
Hunger
Hyperphagia
Inflammation
Insulin
Insulin secretion
Laboratory animals
Lipids
Metabolism
Metabolites
Obesity
Pain
Pandemics
Preferences
Satiety
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Summary:Introduction: Obesity is a pandemic problem associated to development of chronic degenerative diseases. N-arachidonylglycine (NAGly) is speculated to participate in pain regulation, immunity and insulin secretion; some N-acyl amino acids induce hyperphagia; in light of this, NAGly metabolic functions related to energy homeostasis has not been clarified yet. We aim to elucidate the effect of NAGly administration on weight gain, food intake, diet preferences and inflammatory profile changes in a murine model. Methods: 8-week-old BALB/c mice (n=78) were allocated into 4 groups for either sex: control, vector, NAGly-LD (1nM) and NAGly-HD (10nM). NAGly was subcutaneous administered 5 consecutive days for 3 weeks. Mice were exposed to standard diet (SD), high-fat diet (HFD) and high-sugar diet (HSD) simultaneously; weight gain, food intake and diet preferences were evaluated for 21-days. Finally, cytokines were analyzed by enzyme-linked immunosorbent assay (ELISA). Results: final weight gain was found higher in those following NAGly [LD: 21.6±1.1 g and HD: 21.4±2.1 g, (p=0.001)]. NAGly groups consumed more food in the first days; NAGly groups consumed more HFD [5.06±1.60; (p=0.001)] and HSD [1.10±0.69; (p=0.001)]. Conclusion: Our data propose that subcutaneous NAGly promotes acute hyperphagia events. We propose that NAGly might play a role related to the hunger-satiety circuit.
ISSN:2079-052X
2079-0538
DOI:10.9755/ejfa.2021.v33.i3.2657