A crosslinked colloidal network of peptide/nucleic base amphiphiles for targeted cancer cell encapsulation

The use of peptide amphiphiles (PAs) is becoming increasingly popular, not only because of their unique self-assembly properties but also due to the versatility of designs, allowing biological responsiveness, biocompatibility, and easy synthesis, which could potentially contribute to new drug design...

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Bibliographic Details
Published in:Chemical science (Cambridge) 2021-07, Vol.12 (29), p.163-169
Main Authors: Zhou, Yanzi, Qiu, Peng, Yao, Defan, Song, Yanyan, Zhu, Yuedong, Pan, Haiting, Wu, Junchen, Zhang, Junji
Format: Article
Language:English
Subjects:
Adenine
Biocompatibility
Cancer
Chemical compounds
Chemistry
Crosslinking
Design
Encapsulation
Fluorescence
Morphology
Nucleation
Oligonucleotides
Peptides
Rhodamine
Scanning microscopy
Self-assembly
Thymine
Wound healing
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Summary:The use of peptide amphiphiles (PAs) is becoming increasingly popular, not only because of their unique self-assembly properties but also due to the versatility of designs, allowing biological responsiveness, biocompatibility, and easy synthesis, which could potentially contribute to new drug design and disease treatment concepts. Oligonucleotides, another major functional bio-macromolecule class, have been introduced recently as new functional building blocks into PAs, further enriching the tools available for the fabrication of bio-functional PAs. Taking advantage of this, in the present work, two nucleic base-linked (adenine, A and thymine, T) RGD-rich peptide amphiphiles (NPAs) containing the fluorophores naphthalimide and rhodamine ( Nph-A and Rh-T ) were designed and synthesized. The two NPAs exhibit distinctive assembly behaviours with spherical ( Rh-T ) and fibrous ( Nph-A ) morphologies, and mixing Nph-A with Rh-T leads to a densely crosslinked colloidal network ( Nph-A / Rh-T ) via mutually promoted supramolecular polymerization via nucleation-growth assembly. Because of the RGD-rich sequences in the crosslinked network, further research on in situ targeted cancer cell (MDA-MB-231) encapsulation via RGD-integrin recognition was performed, and the modulation of cell behaviours ( e.g. , cell viability and migration) was demonstrated using both confocal laser scanning microscopy (CLSM) imaging and a scratch wound healing assay. A cross-linking of peptide-nucleic base amphiphiles leads to a dense colloidal network that can perform targeted cancer cell encapsulation in situ .
ISSN:2041-6520
2041-6539
DOI:10.1039/d1sc02995a