Fast, simple and reliable triglyceride composition analysis of milk fat for discrimination of cheese origin and adulteration detection

Optimization of a novel high performance liquid chromatography with refractive index detection method, together with accelerated solvent extraction for determining the triglyceride composition of lipids to enable rapid discrimination of goat and cow cheeses is presented. The method was applied to de...

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Bibliographic Details
Published in:IOP conference series. Earth and environmental science 2019-09, Vol.333 (1), p.12094
Main Authors: Petronijevic, R B, Trbovic, D, Milicevic, D
Format: Article
Language:English
Subjects:
Animal fat
Cheese
Composition
Dairy products
Discrimination
Goats
High performance liquid chromatography
Lipids
Liquid chromatography
Milk
Oils & fats
Optimization
Principal components analysis
Refractivity
Reliability analysis
Solvent extraction
Triglycerides
Vegetable oils
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Summary:Optimization of a novel high performance liquid chromatography with refractive index detection method, together with accelerated solvent extraction for determining the triglyceride composition of lipids to enable rapid discrimination of goat and cow cheeses is presented. The method was applied to determine adulteration of goat and cow cheeses with vegetable oils/fats, to distinguish cheese analogues from natural cheeses, and to determine the amounts of palm fat added to cheese. Principal component analysis of triglycerides grouped by equivalent carbon number was used for confirmation of cheeses' animal origins. A total of 130 cheeses or cheese analogues were included in this study. The method was calibrated in the range of 0 to 50 % palm fat in milk fat, with recovery values being from 46.50 % to 108.48 %. The content of palm fat found in adulterated cheese was from 10 to 16 %, and 5 to 10 % in cow and goat cheeses, respectively. Due to the rapidity of the method, employment of relatively inexpensive equipment and the reliability of the results, the method proved to be suitable for analytical laboratories that are required to process large numbers of cheese samples in a short time.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/333/1/012094