The Expression of Hoxa-10 Gene Among Stunted and Normal Stature Children

Background/Aims: Hoxa-10 gene plays key roles in embryonic development and supports bone formation has known in many research. As the consequence of its function, the expression of hoxa-10 gene among children may differ according to their growth rate. This study was addressed to answer whether the e...

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Bibliographic Details
Published in:Annals of nutrition and metabolism 2019-01, Vol.75, p.68
Main Authors: Sumarmi, Sri, Farapti, Aksono, E Bimo
Format: Article
Language:English
Subjects:
Antisense RNA
Blood cells
Bone growth
Children
Children & youth
Childrens health
Deoxyribonucleic acid
DNA
Embryogenesis
Embryonic growth stage
Gene banks
Gene expression
Genotype & phenotype
Growth rate
Homology
Human growth
Nucleotides
Nutrition
Osteogenesis
Pediatrics
Polymerase chain reaction
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Summary:Background/Aims: Hoxa-10 gene plays key roles in embryonic development and supports bone formation has known in many research. As the consequence of its function, the expression of hoxa-10 gene among children may differ according to their growth rate. This study was addressed to answer whether the expression of Hoxa-10 gene differ among stunted and normal growth children. Methods: Hoxa-10 gene was obtained by DNA extraction from venous blood sample from stunted children and normal growth children. Analysis of hoxa-10 gene was carried using polymerase chain reaction (PCR) methods. Optimation analysis depicted that the target gene was found in 395 base pairs. Homologue analysis was carried on the DNA squencing to identify profile of nucleotide of the gene among children by comparing with homo sapiens Hoxa-10 antisense RNA (Hoxa-10-AS), in gene bank ID NR046609. Results: Base on PCR analysis, it showed that the expression of Hoxa-10 gene in blood cells was lower among stunted children compare to normal growth children. In addition, homologue analysis depicted that profile of nucleotide among stunted children has lower similarity (95.4%) with the gene bank compare to normal stature children (96%). Conclusion: It is indicate that the difference expression of Hoxa-10 gene between stunted and normal growth children was due to any change on nucleotide profile.
ISSN:0250-6807
1421-9697
DOI:10.1159/000501751