A CALAFATE EXTRACT IMPROVES THE GLYCIDIC PROFILE IN MICE WITH OBESITY INDUCED BY HIGH FAT DIET

Background: Since in the etiology of obesity a strong component of oxidative stress and inflammation has been described, it is relevant to study foods with antioxidant and/or anti-inflammatory properties, such as calafate, a Chilean native fruit rich in polyphenols. Objective: To evaluate the effect...

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Bibliographic Details
Published in:Annals of nutrition and metabolism 2020-01, Vol.76, p.87
Main Authors: Duarte, L, Villanueva, V, Jimenez, D, Uribe, D, Garcia-Diaz, D F
Format: Article
Language:English
Subjects:
Adipose tissue
Adipose tissue (brown)
Anti-inflammatory agents
Antioxidants
Blood glucose
Body fat
Body weight gain
Calorimetry
Cytokines
Diet
Dosage
Energy expenditure
Etiology
Glucose tolerance
High fat diet
Inflammation
Insulin
Interleukin 6
Monocyte chemoattractant protein 1
Nutrition
Obesity
Oils & fats
Oxidative stress
Polyphenols
Rodents
Serum levels
Statistical analysis
Tumor necrosis factor-α
Variance analysis
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Summary:Background: Since in the etiology of obesity a strong component of oxidative stress and inflammation has been described, it is relevant to study foods with antioxidant and/or anti-inflammatory properties, such as calafate, a Chilean native fruit rich in polyphenols. Objective: To evaluate the effect of the treatment of a calafate extract in mice with obesity induced by high fat diet. Methods: 25 C57BL6 mice of 8 weeks of age were divided into 3 dietary regimens or treatments: Control (n=5), High fat diet (n=10) and High Fat Diet + calafate (n=10). All groups received food and water ad libitum during 4 months. After 3 months, the High Fat Diet + calafate group was treated with a daily dose of 50 mg total polyphenols per kg of weight of calafate extract rich in polyphenols (added to water) for 3 weeks. After 3.5 months, an intraperitoneal glucose tolerance test (IPGTT) and indirect calorimetry were performed. At the fourth month the animals were sacrificed. Final body weights were recorded, and serum was isolated. In addition, interscapular Brown Adipose Tissue (BAT) and epididymal White Adipose Tissue (WAT) were obtained. Serum insulin levels were determined through ELISA. Serum levels of inflammatory cytokines MCP-1, TNF-α and IL6, were evaluated through Luminex kit. Statistical analysis was performed using ANOVA. Results: Mice with a High Fat Diet had a significantly greater weight gain than controls (p
ISSN:0250-6807
1421-9697