Long-Chain Free Fatty Acids Influence Lipid Accumulation, Lysosome Activation and Glycolytic Shift in Various Cells In Vitro

Hydrophobic molecules may be toxic when present in excess. When dissolved in membranes, hydrophobic molecules disrupt membrane function. Studies on the effects of free fatty acids (FFA) on cultured cells contradict each other. Here we describe the effects of FFA on various human cells in culture. Th...

Full description

Saved in:
Bibliographic Details
Published in:Molecular biology (New York) 2021-07, Vol.55 (4), p.624-636
Main Authors: Vishnyakova, K. S., Popov, K. V., Pan, X., Jasko, M. V., Yegorov, Y. E.
Format: Article
Language:English
Subjects:
Autophagy
Biochemistry
Biomedical and Life Sciences
Cell culture
Fatty acids
Glycolysis
Hepatocytes
Human Genetics
Hydrophobicity
Life Sciences
Molecular Cell Biology
Oxidative phosphorylation
Oxygen consumption
Phagocytosis
Phosphorylation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Hydrophobic molecules may be toxic when present in excess. When dissolved in membranes, hydrophobic molecules disrupt membrane function. Studies on the effects of free fatty acids (FFA) on cultured cells contradict each other. Here we describe the effects of FFA on various human cells in culture. The addition of long-chain FFA (oleic, palmitic, linoleic, linolenic, etc.) to cultured cells led to lipid accumulation in hepatocytes and muscle cells, initiation of autophagy, and uncoupling of oxidative phosphorylation. Although treated cells increase their oxygen consumption, metabolic shifts in favor of glycolysis were observed. All these effects were expressed to varying degrees in different cells and with the addition of different FFAs. The mechanisms of these FFA effects are discussed, as well their practical implications.
ISSN:0026-8933
1608-3245
DOI:10.1134/S0026893321030146