Fusion of clathrin and caveolae endocytic vesicles revealed by line-switching dual-color STED microscopy

Clathrin- and caveolae-mediated endocytosis are the most commonly used pathways for the internalization of cell membrane receptors. However, due to their dimensions are within the diffraction limit, traditional fluorescence microscopy cannot distinguish them and little is known about their interacti...

Full description

Saved in:
Bibliographic Details
Published in:Journal of innovative optical health science 2021-11, Vol.14 (6), p.2150017-1-2150017-10
Main Authors: Ruan, Hefei, Yu, Jianqiang, Wu, Yayun, Tang, Xiaojun, Yuan, Jinghe, Fang, Xiaohong
Format: Article
Language:English
Subjects:
Beads
Caveolae
Cell membranes
Clathrin
Color
Crosstalk
Depletion
dual-color
Endocytosis
Fluorescence
Fluorescence microscopy
Image resolution
Internalization
line-switching
Microscopy
Protein interaction
Receptors
Research Article
Scanning
Side effects
sted
Stimulated emission
super-resolution microscopy
Switching
Vesicles
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Clathrin- and caveolae-mediated endocytosis are the most commonly used pathways for the internalization of cell membrane receptors. However, due to their dimensions are within the diffraction limit, traditional fluorescence microscopy cannot distinguish them and little is known about their interactions underneath cell membrane. In this study, we proposed the line-switching scanning imaging mode for dual-color triplet-state relaxation (T-Rex) stimulated emission depletion (STED) super-resolution microscopy. With this line-switching mode, the cross-talk between the two channels, the side effects from pulse picker and image drift in frame scanning mode can be effectively eliminated. The dual-color super-resolution imaging results in mixed fluorescent beads validated the excellent performance. With this super-resolution microscope, not only the ring-shaped structure of clathrin and caveolae endocytic vesicles, but also their semi-fused structures underneath the cell membrane were distinguished clearly. The resultant information will greatly facilitate the study of clathrin- and caveolae-mediated receptor endocytosis and signaling process and also our home-built dual-color T-Rex STED microscope with this line-switching imaging mode provides a precise and convenient way to study subcellular-scale protein interactions.
ISSN:1793-5458
1793-7205
DOI:10.1142/S1793545821500176