Shining light on anther culture, a poorly understood regeneration route in passion fruit (Passiflora gibertii N. E. Brown): histological, hormonal, and phytochemical aspects

Plant regeneration from reproductive organs is a poorly understood route in passion fruit. The present study provides a morpho-hystological description of somatic embryogenesis from anther culture in Passiflora gibertii N. E. Br . , as well as a phytochemical profile and genetic analysis of ploidy i...

Full description

Saved in:
Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 2021-12, Vol.57 (6), p.998-1008
Main Authors: dos Santos Soares, Wellington, Bruckner, Cláudio Horst, da Cruz, Ana Cláudia Ferreira, Rocha, Diego Ismael, de Matos, Elyabe Monteiro, Koehler, Andréa Dias, Viccini, Lyderson Facio, Otoni, Wagner Campos
Format: Article
Language:English
Subjects:
Anthers
Biomedical and Life Sciences
Cell Biology
Cell culture
Cell division
Developmental Biology
Diploids
Embryonic growth stage
Embryos
Flow cytometry
Flowers & plants
Fruits
Genetic engineering
Genomes
Life Sciences
Passion fruit
Pectin
Physiology
Phytochemicals
Plant Breeding/Biotechnology
Plant Genetics and Genomics
Plant Sciences
Plant Tissue Culture
Ploidy
Regeneration
Somatic embryogenesis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Plant regeneration from reproductive organs is a poorly understood route in passion fruit. The present study provides a morpho-hystological description of somatic embryogenesis from anther culture in Passiflora gibertii N. E. Br . , as well as a phytochemical profile and genetic analysis of ploidy in the generated embryos. Flower buds were collected during the pre-anthesis period. Murashige and Skoog culture medium was supplemented with 2,4-dichlorophenoxyacetic acid and 6-benzyladenine. Embryogenic calluses were collected at different ages for histological, biochemical, and genetic evaluation of their ploidy. Anatomical characterization revealed the cell layers of the epidermal tissue involved in morphogenic events. Specifically, regions of intense cell division led to embryo formation and its subsequent regeneration. Moreover, as shown by hormonal and secondary compounds’ profiles, these regions were characterized by the presence of reserve compounds and higher levels of proteins and pectins. Finally, flow cytometry confirmed the diploid nature of the embryos. The present results define a pioneering study of anthers in P. gibertii N. E. Brown, and could serve as a blueprint for future in vitro regeneration protocols.
ISSN:1054-5476
1475-2689
DOI:10.1007/s11627-021-10179-z