Pre-centrifugation before DNA extraction mitigates extraction efficiency reduction of environmental DNA caused by the preservative solution (benzalkonium chloride) remaining in the filters

In the biomonitoring using environmental DNA (eDNA) analysis, the suppression of eDNA degradation has been an important issue to estimate accurately the presence of target species and their eDNA concentrations. A recently proposed eDNA preservation technique requires only the addition of benzalkoniu...

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Bibliographic Details
Published in:Limnology 2022-01, Vol.23 (1), p.9-16
Main Authors: Tsuji, Satsuki, Nakao, Ryohei, Saito, Minoru, Minamoto, Toshifumi, Akamatsu, Yoshihisa
Format: Article
Language:English
Subjects:
Benzalkonium chloride
Biomedical and Life Sciences
Biomonitoring
Centrifugation
Centrifuging
Chlorides
Deoxyribonucleic acid
DNA
Ecology
Environment
Environmental DNA
Filters
Filtration
Freshwater & Marine Ecology
Life Sciences
Preservatives
Rapid Communication
Reduction
Water analysis
Water purification
Water sampling
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Summary:In the biomonitoring using environmental DNA (eDNA) analysis, the suppression of eDNA degradation has been an important issue to estimate accurately the presence of target species and their eDNA concentrations. A recently proposed eDNA preservation technique requires only the addition of benzalkonium chloride (BAC) to water samples and has been used for various studies as an alternative solution to on-site filtration. However, BAC remaining in filters may adsorb to and inhibit the function of the enzyme used to extract eDNA from filters. In this study, we tested whether eDNA yield is affected by BAC addition immediately before filtration and demonstrated that the BAC in water remaining in filters decreased the eDNA yield significantly. We found that simple pre-centrifugation is effective for removing the remaining water from the filters to prevent the eDNA yield reduction due to the use of BAC. This is the most important finding in the present study. Appropriate eDNA extraction techniques are needed when BAC is used to secure eDNA yields and expand the application of eDNA analysis for biomonitoring.
ISSN:1439-8621
1439-863X
DOI:10.1007/s10201-021-00676-w