Diaphorina communis: Molecular identification, development on Citrus reticulata, and acquisition and transmission of ‘Candidatus Liberibacter asiaticus’

• Published in: Journal of applied entomology (1986) 2022-02, Vol.146 (1-2), p.118-129
• Main Authors: Om, Namgay, Beattie, George Andrew Charles, Donovan, Nerida J., Holford, Paul
• Format: Article
• Language: English
• Subjects: Adults; Bergera koenigii; Candidatus Liberibacter asiaticus; Citrus fruits; Citrus reticulata; curry leaf; Diaphorina citri; Diaphorina communis; Fruits; HLB; Host plants; Insects; Leaves; Mandarins; Nymphs; Oviposition; Pathogens; Phylogeny; psyllid; Seedlings; Survival
• ISSN: 0931-2048; 1439-0418
• DOI: 10.1111/jen.12937

The black curry leaf psyllid, Diaphorina communis, is a host of the citrus pathogen, ‘Candidatus Liberibacter asiaticus’ (‘CLas’). However, there is a paucity of information on its biology; hence, this study was conducted to evaluate survival and development on citrus, in this instance mandarin (Citrus reticulata) and curry leaf (Bergera koenigii), and transmission of ‘CLas’. Given its similarity with the Asian citrus psyllid (Diaphorina citri), sequences of the COI and 16S genes were examined to see if they could distinguish molecularly between nymphs of these two species. Field and laboratory experiments showed that D. communis nymphs transferred to mandarin branches failed to survive. Adults could survive on flush growth of ‘CLas’‐infected mandarin trees; however, little oviposition took place, and the single resulting nymph did not survive to the second instar. These observations suggest that D. communis does not develop, or rarely develops on mandarin and possibly other Citrus spp. Curry leaf appears to be its preferred host, and complete development can take place, possibly exclusively, on this species. Studies on acquisition of ‘CLas’ by D. communis and possible transmission assessed in 2014 and 2015 using greenhouse‐grown curry leaf and mandarin seedlings with naturally infected mandarin stumps as a source of ‘CLas’ indicated a low acquisition rate of ‘CLas’. In addition, although D. communis can acquire ‘CLas’, it either cannot transmit it or transmission is limited, and the pathogen may not multiply within the insect. Phylogenetic analyses indicate that differences in COI and 16S regions may prove useful for differentiating between early instar D. communis and D. citri nymphs when fifth instar nymphs and adults are not present on host plants.