Productive biofilms of nitrile hydrolyzing bacteria

Biocatalysts were obtained on the basis of biofilms of nitrile-utilizing bacteria Rhodococcus ruber gt1, R. erythropolis 11-2, Pseudomonas fluorescens C2, and Alcaligenes faecalis 2 and the respective potentials were estimated. The objective of this work was to use biofilms of nitrile-utilizing bact...

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Bibliographic Details
Main Authors: Maksimova, Yu. G., Zorina, A. S., Demakov, V. A.
Format: Conference Proceeding
Language:English
Subjects:
Acrylamide
Acrylic acid
Acrylics
Bacteria
Biofilms
Biotransformation
Mineral fibers
Pseudomonas fluorescens
Rhodococcus
Substrates
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Summary:Biocatalysts were obtained on the basis of biofilms of nitrile-utilizing bacteria Rhodococcus ruber gt1, R. erythropolis 11-2, Pseudomonas fluorescens C2, and Alcaligenes faecalis 2 and the respective potentials were estimated. The objective of this work was to use biofilms of nitrile-utilizing bacteria for acrylonitrile and acrylamide conversion and determine their ability for biotransformation that can be used in productive biocatalysis. Our study concluded that biofilms of R. ruber gt1 grown on mineral fibers produced 40% acrylamide solution from acrylonitrile at the fractional introduction of the substrate and can be considered as productive biofilms, and A. faecalis biofilms synthesized 2.5% acrylic acid from acrylamide. P. fluorescens C2 biofilms and A. faecalis 2 biofilms and planktonic cells have used acrylic acid in further metabolic pathways, but in case of R. erythropolis 11-2 the accumulation of acrylic acid was a dead-end pathway.
ISSN:0094-243X
1551-7616
DOI:10.1063/5.0069399