Analysis of acrylamide in vegetable chips after derivatization with 2-mercaptobenzoic acid by liquid chromatography–mass spectrometry

Since many years, acrylamide (AA) is a well-known toxicologically relevant processing contaminant (“food-borne toxicant”). However, only during the recent years, high levels of acrylamide have been reported in vegetable chips. In the present study, AA was quantitated via a modified derivatization pr...

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Bibliographic Details
Published in:European food research & technology 2022-04, Vol.248 (4), p.937-946
Main Authors: Oellig, Claudia, Gottstein, Eva, Granvogl, Michael
Format: Article
Language:English
Subjects:
Acrylamide
Agriculture
Analytical Chemistry
Biotechnology
Cation exchanging
Chemistry
Chemistry and Materials Science
Chromatography
Contaminants
Dilution
Food contamination
Food processing
Food Science
Forestry
Hexanes
Ions
Liquid chromatography
Mass spectrometry
Mass spectroscopy
n-Hexane
Original Paper
Potatoes
Quantitation
Radiochemical analysis
Scientific imaging
Solid phases
Spectroscopy
Stable isotopes
Toxicants
Vegetables
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Summary:Since many years, acrylamide (AA) is a well-known toxicologically relevant processing contaminant (“food-borne toxicant”). However, only during the recent years, high levels of acrylamide have been reported in vegetable chips. In the present study, AA was quantitated via a modified derivatization procedure with 2-mercaptobenzoic acid based on stable isotope dilution analysis and liquid chromatography–mass spectrometry. Extraction with a modified QuEChERS (quick, easy, cheap, efficient, rugged, safe) method, defatting with n -hexane, and a solid phase extraction clean-up with strong cation-exchange material were performed prior to the derivatization step. Limits of detection and quantitation (LoD and LoQ) were 12 and 41 µg of AA/kg of vegetable chips (estimated via signal-to-noise ratios of 3:1 and 10:1, respectively), and thus below the LoQ of 50 µg/kg requested by the European Food Safety Authority. Recovery rates between 92 and 101% at four spiking levels with a good precision expressed as a relative standard deviation  
ISSN:1438-2377
1438-2385
DOI:10.1007/s00217-021-03898-5