Temporal Variation in the Microbiome of Tropical and Temperate Octocorals

• Published in: Microbial ecology 2022-05, Vol.83 (4), p.1073-1087
• Main Authors: Haydon, Trent D., Suggett, David J., Siboni, Nachshon, Kahlke, Tim, Camp, Emma F., Seymour, Justin R.
• Format: Article
• Language: English
• Subjects: Animals; Anthozoa - microbiology; Bacteria; Bacteria - genetics; Biodiversity; Biomedical and Life Sciences; Community structure; Coral Reefs; Corals; Ecology; Endozoicomonas; Gammaproteobacteria - genetics; Geoecology/Natural Processes; Host Microbe Interactions; Life Sciences; Marine invertebrates; Microbial Ecology; Microbiology; Microbiomes; Microbiota; Microorganisms; Nature Conservation; Reefs; RNA, Ribosomal, 16S - genetics; Roles; rRNA 16S; Sarcophyton; Sinularia; Species; Summer; Temporal variations; Tropical climate; Tropical environment; Tropical environments; Water Quality/Water Pollution; Winter
• ISSN: 0095-3628; 1432-184X
• DOI: 10.1007/s00248-021-01823-7

Bacterial members of the coral holobiont play an important role in determining coral fitness. However, most knowledge of the coral microbiome has come from reef-building scleractinian corals, with far less known about the nature and importance of the microbiome of octocorals (subclass Octocorallia), which contribute significantly to reef biodiversity and functional complexity. We examined the diversity and structure of the bacterial component of octocoral microbiomes over summer and winter, with a focus on two temperate ( Erythropodium hicksoni , Capnella gaboensis ; Sydney Harbour) and two tropical ( Sinularia sp., Sarcophyton sp.; Heron Island) species common to reefs in eastern Australia. Bacterial communities associated with these octocorals were also compared to common temperate ( Plesiastrea versipora ) and tropical ( Acropora aspera ) hard corals from the same reefs. Using 16S rRNA amplicon sequencing, bacterial diversity was found to be heterogeneous among octocorals, but we observed changes in composition between summer and winter for some species ( C. gaboensis and Sinularia sp.), but not for others ( E. hicksoni and Sarcophyton sp.). Bacterial community structure differed significantly between all octocoral species within both the temperate and tropical environments. However, on a seasonal basis, those differences were less pronounced. The microbiomes of C. gaboensis and Sinularia sp. were dominated by bacteria belonging to the genus Endozoicomonas , which were a key conserved feature of their core microbiomes. In contrast to previous studies, our analysis revealed that Endozoicomonas phylotypes are shared across different octocoral species, inhabiting different environments. Together, our data demonstrates that octocorals harbour a broad diversity of bacterial partners, some of which comprise ‘core microbiomes’ that potentially impart important functional roles to their hosts.