Impact of culture vessel ventilation on the anatomy and morphology of micropropagated carnation

Dianthus caryophyllus cv. Nelken was cultured in vitro under different ventilation rates (0.11, 0.21, 0.68 and 0.86 changes h−1). Ventilation modified the anatomical characteristics of shoots and leaves described for plants grown in non-ventilated vessels: the cuticle became thicker, there was a dec...

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Bibliographic Details
Published in:Plant cell, tissue and organ culture tissue and organ culture, 2000-01, Vol.63 (3), p.207-214
Main Authors: MAJADA, J. P, TADEO, F, FAL, M. A, SANCHEZ-TAMES, R
Format: Article
Language:English
Subjects:
Acclimatization
Biological and medical sciences
Biotechnology
Cell culture
Cell size
Cell walls
Dianthus caryophyllus
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
In vitro propagation: entire plant regeneration from tissues and cell cultures
Methods. Procedures. Technologies
Phenotypes
Photosynthesis
Plant cells and fungal cells
Shoots
Ventilation
Vessels
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Summary:Dianthus caryophyllus cv. Nelken was cultured in vitro under different ventilation rates (0.11, 0.21, 0.68 and 0.86 changes h−1). Ventilation modified the anatomical characteristics of shoots and leaves described for plants grown in non-ventilated vessels: the cuticle became thicker, there was a decreased cell size and intracellular space size. Also, there were more photosynthetic and supportive tissues, including thicker cell walls. Increased ventilation promoted in vitro hardening of micropropagated carnation shoots, and pushed the culture-induced phenotype closer to that of ex vitro acclimatized plants. Anatomical variability of in vitro-grown plants was demonstrated to be a consequence of ventilation.
ISSN:0167-6857
1573-5044
DOI:10.1023/A:1010650131732