Strain Klebsiella ZP-2 inoculation activating soil nutrient supply and altering soil phosphorus cycling

• Published in: Journal of soils and sediments 2022-08, Vol.22 (8), p.2146-2157
• Main Authors: Liu, Yaohui, Hosseini Bai, Shahla, Wang, Jiawei, Hu, Dongnan, Wu, Ruohong, Zhang, Wenyuan, Zhang, Manyun
• Format: Article
• Language: English
• Subjects: Bacteria; Bicarbonates; Biofertilizers; Cycles; Earth and Environmental Science; Environ Risk Assess; Environment; Environmental Physics; Fertility; Fertilizers; Genes; Hydrochloric acid; Hydroxides; Inoculation; Klebsiella; Microbiological strains; Nutrient cycles; Phosphatase; Phosphates; Phosphorus; rRNA 16S; Sec 2 • Global Change; Sodium; Sodium bicarbonate; Sodium hydroxide; Soil bacteria; Soil fertility; Soil improvement; Soil microorganisms; Soil nutrients; Soil properties; Soil Science & Conservation; Soils; Sustainable Land Use • Research Article
• ISSN: 1439-0108; 1614-7480
• DOI: 10.1007/s11368-022-03221-z

Purpose Phosphorus (P) plays an important role in enhancing plant yield. Phosphate-solubilizing bacteria (PSB) could enhance soil P availability. This study aimed to determine the effects of PSB inoculation on soil P fractions and bacterial community and reveal the comprehensive linkages among soil bacteria, P fractions, and nutrient contents. Materials and methods In this study, PSB, Klebsiella ZP-2, was inoculated into soil with four different inoculation rates (2%, 4%, 6%, and 8%, bacterial suspension/soil weight = v:w); soil phosphatase activity, P fractions, function gene, and 16S rRNA were detected to comprehensively assess the effects of strain ZP-4 on soil properties, P fractions, and bacterial community. Results and discussion Soil available P and N were significantly higher in the treatments with 4% and 6% inoculation rates than that in the control. Compared with control, the contents of soil inorganic P extracted by sodium hydroxide (NaOH-Pi), water (H 2 O-Pi), and sodium bicarbonate (NaHCO 3 -Pi) were significantly increased. However, inorganic P extracted by hydrochloric acid (HCl-Pi) and residual P (residual-P) contents were significantly decreased, especially in the 4% inoculation rates. The strain ZP-2 stimulated functional genes ( pho C and pho D) and soil phosphatase activity to active soil P. Meanwhile, the bacterial community structures were also significantly changed following the strain ZP-2 inoculation. Conclusion The strain ZP-2 inoculation improved soil available N and P contents, stimulated phosphatase activity to accelerate P cycling, and altered soil bacterial community. The ZP-2 strain has the potential to be used as a biofertilizers to improve soil fertility.