In situ intracellular Raman spectroscopic detection with graphene-based thermoelectric optical tweezers

Label-free Raman spectroscopic detection of cellular elements is an important technique for biological science applications; however, the implementation of proactive intracellular detection remains a challenge. In this work, we demonstrate a graphene-based thermoelectric optical tweezers system for...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2022-06, Vol.361, p.131722, Article 131722
Main Authors: Deng, Ruping, Zhang, Yuquan, Wang, Xianyou, Xie, Xi, Song, Yuan, Bu, Jing, Min, Changjun, Yuan, Xiaocong
Format: Article
Language:English
Subjects:
Graphene
Graphene substrate
Intracellular detection
Nanoparticles
Photothermal conversion
Raman spectroscopy
Spectroscopy
Substrates
Thermoelectric optical tweezers
Thermoelectricity
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Summary:Label-free Raman spectroscopic detection of cellular elements is an important technique for biological science applications; however, the implementation of proactive intracellular detection remains a challenge. In this work, we demonstrate a graphene-based thermoelectric optical tweezers system for in situ intracellular Raman spectral detection on-a-chip applications. Because of the excellent photothermal conversion effect of the graphene substrate, a potent thermoelectric effect is generated to not only strengthen the trap for biological cells, but also accelerate metallic nanoparticles to permeate into these cells for intracellular Raman enhancement. An in situ intracellular Raman spectrum is thus acquired that offers the opportunity to distinguish the intracellular elements. This work provides a promising approach for use in cytobiology research and is expected to enable exciting new applications in the detection of complex intracellular life processes. •In situ Raman spectroscopic detection of intracellular elements is implemented both in theory and experiment.•An on-chip graphene-based thermoelectric optical tweezer system is constructed.•The potent thermoelectric effect attracts metallic nanoparticles to permeate into the trapped cell.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2022.131722