A bivalent aptamer and terminus-free siRNA junction nanostructure for targeted gene silencing in cancer cells

Small interfering RNA (siRNA) has increasingly evolved as a potent therapeutic solution for several pathological conditions including cancers via post-transcriptional oncogene suppression in cellular pathways. And, the key for siRNA-based therapy highly relies on the successful siRNAs delivery into...

Full description

Saved in:
Bibliographic Details
Published in:Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2022-10, Vol.1 (4), p.8315-8321
Main Authors: Yang, Fang, Li, Shunmei, Yuan, Ruo, Xiang, Yun
Format: Article
Language:English
Subjects:
Apoptosis
Aptamers
Cancer
Gene sequencing
Gene silencing
Nanostructure
Post-transcription
Ribonucleic acid
RNA
Self-assembly
siRNA
Synthesis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Small interfering RNA (siRNA) has increasingly evolved as a potent therapeutic solution for several pathological conditions including cancers via post-transcriptional oncogene suppression in cellular pathways. And, the key for siRNA-based therapy highly relies on the successful siRNAs delivery into the target cells, which is significantly challenged by their instability, poor cellular uptake and targeting capability. To overcome these issues, herein, a new type of RNA nanostructure, the bivalent aptamer and terminus-free siRNA junction, is synthesized and employed for effective gene silencing in cancer cells. Such a siRNA junction can be readily prepared by the self-assembly of three RNA sequences and subsequent ligation of the nicks. The as-synthesized siRNA junction shows highly improved enzymatic stability and targeting capability and can be efficiently delivered into the target cells to induce cell apoptosis. With these integrated advantages, the siRNA junction can therefore offer new potentials for the design of different siRNA therapeutics for various diseases. Specific and efficient gene silencing in cancer cells is achieved by a bivalent aptamer and terminus-free siRNA junction.
ISSN:2050-750X
2050-7518
DOI:10.1039/d2tb01414a