A UV-Vis Spectroscopic Study of the Auto-Oxidation of Adrenaline

The auto-oxidation of adrenaline in an alkaline medium (pH 10.65) in the presence of sulfite was studied by UV-Vis spectroscopy. The absorption spectra of the final auto-oxidation product (λ m = 290–300 nm, ε m = 8400 M –1 ·cm –1 ), the intermediate adduct of adrenochrome with sulfite (λ m =343 nm,...

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Bibliographic Details
Published in:Journal of applied spectroscopy 2022-11, Vol.89 (5), p.905-912
Main Authors: Ivanova, M. A., Nefedova, E. K., Mal’tseva, E. M., Kalensii, A. V., Zvekov, A. A.
Format: Article
Language:English
Subjects:
Absorption spectra
Analytical Chemistry
Atomic/Molecular Structure and Spectra
Carbonyl compounds
Carbonyls
Chemical reaction, Rate of
Data processing
Oxidation
Oxidation-reduction reaction
Phenols
Physics
Physics and Astronomy
Spectrum analysis
Sulfites
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Summary:The auto-oxidation of adrenaline in an alkaline medium (pH 10.65) in the presence of sulfite was studied by UV-Vis spectroscopy. The absorption spectra of the final auto-oxidation product (λ m = 290–300 nm, ε m = 8400 M –1 ·cm –1 ), the intermediate adduct of adrenochrome with sulfite (λ m =343 nm, ε m = 68,000 M –1 ·cm –1 ), and a reactive intermediate, presumably adrenolutin (λ m = 311 nm, ε m = 140,000 M –1 ·cm –1 ), were identified using experimental data processing. The estimated equilibrium constant for the formation of the adrenochrome adduct with sulfite (5000 M –1 ) agrees with the literature data for carbonyl compounds. These results account for the contradictions in the data obtained by various authors concerning the oxidation and auto-oxidation of adrenaline in alkaline media.
ISSN:0021-9037
1573-8647
DOI:10.1007/s10812-022-01446-x