Engineering Photocleavable Protein-decorated Hydrogels to Regulate Cell Adhesion and Migration

Cell adhesion and migration play essential roles in tissue development and maintenance, and abnormal cell migration is involved in life-threatening diseases, including vascular disease, tumor formation, and metastasis. The advances in hydrogel-based 3D cell culture development facilitated the invest...

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Published in:Chemical research in Chinese universities 2022-12, Vol.38 (6), p.1512-1517
Main Authors: Li, Tianyi, Huang, Yaying, Lu, Chenjing, Gu, Liwen, Cao, Yi, Yin, Sheng
Format: Article
Language:English
Subjects:
Analytical Chemistry
Binding
Cell adhesion
Cell adhesion & migration
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Controllability
Decoration
Hydrogels
In vitro methods and tests
In vivo methods and tests
Inorganic Chemistry
Light
Organic Chemistry
Physical Chemistry
Proteins
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Summary:Cell adhesion and migration play essential roles in tissue development and maintenance, and abnormal cell migration is involved in life-threatening diseases, including vascular disease, tumor formation, and metastasis. The advances in hydrogel-based 3D cell culture development facilitated the investigation of cell motility behavior, including cell-cell and cell-matrix adhesion and cell migration in a microenvironment more related to in vivo situations. Establishing advanced methods for these in vitro studies is thus necessary. Photo-sensitive proteins show advantages in remote and non-invasive regulation of hydrogels’ properties, and thus are of great potential in regulating 3D cultured cells’ behavior. In the presented study, we engineered photocleavable protein(PhoCl)-decorated hydrogels to regulate cell adhesion and migration of MDA-MB-231. The integrin-binding motif RGD was fused to the PhoCl and was decorated on the hydrogel. After being exposed to light at 405 nm, the PhoCl was cleaved and the RGD motif was released, resulting in detachment of the binding cells. The regulatory effect of the light illumination showed a time-dependent and cell density-dependent manner. Furthermore, the elimination of RGD by patterned light exposure completely suspended the cell migration to the corresponding region, suggesting a controllable regulation of the cell migration direction.
ISSN:1005-9040
2210-3171
DOI:10.1007/s40242-022-2097-7