Red LED light and different cultivation methods changed the essential oil composition of Acmella oleracea

• Published in: Plant cell, tissue and organ culture tissue and organ culture, 2022-12, Vol.151 (3), p.511-520
• Main Authors: Dedino, Diego Baraldi, de Lima, Julliane Destro, Bortolucci, Wanessa de C., Rivadavea, Wesley R., Lovato, Evellyn C. W., Gazim, Zilda C., Gonçalves, José E., Monzon, Daisy L. R., da Silva, Glacy J.
• Format: Article
• Language: English
• Subjects: Acmella oleracea; Biochemical oxygen demand; Biomedical and Life Sciences; Cadinol; Chemical composition; Cultivation; Design of experiments; Essential oils; Experimental design; Gas chromatography; Life Sciences; Light emitting diodes; Mass spectrometry; Mass spectroscopy; Metabolites; Oils & fats; Original Article; Photosynthesis; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Secondary metabolites
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-022-02367-5

The secondary metabolism of plants is affected by several factors, including the type of cultivation and light, which directly interfere with photosynthesis, causing changes in the content and composition of secondary plant metabolites. Thus, this study sought to answer whether the light lengths of red light emitting diodes (LED), as well as different cultivation methods, can cause changes in the chemical composition of the essential oil of Acmella oleracea . The experimental design was divided into four treatments. T1: in vitro using LED lights for indoor cultivation; T2: in vitro with red LED light; T3: in pot with LED lights for indoor cultivation and T4: in pot with red LED light. All treatments were kept in a biochemical oxygen demand oven for 45 days. Leaf material was collected, essential oil was extracted by hydrodistillation, with subsequent chemical characterization by gas chromatography coupled with mass spectrometry (GC/MS). The results showed that trans -cariophyllene (13.04% and 8.89%) and 2-tridecanone (16.45% and 15.09%) compounds were directly affected by red LED light, in both in vitro (T2) and in pot (T4) treatments. The major compound α-cadinol (18.75%) and 4-epi-cubedol (29.11%) were directly affected by the treatment T3. The results indicated that red light and cultivation methods significantly influenced the chemical composition of A . oleracea essential oil. Key message Red LED light affected the production of secondary metabolites in Acmella oleracea . Trans -cariophyllene and 2-tridecanone were affected by red LED light in both cultivation treatments.