Jasmonic acid: effects on growth and development of isolated tomato roots cultured in vitro

The effect of jasmonic acid (JA) on growth, morphology, and development of roots and the possible interaction of JA with ethylene were investigated using isolated tomato roots (Lycopersicon esculentum Mill. cv Bush Beefsteak) cultured in vitro. JA had a number a direct effects on the growth and deve...

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Bibliographic Details
Published in:International journal of plant sciences 1996-11, Vol.157 (6), p.713-721
Main Authors: Tung, P. (University of Calgary, Calgary, Alberta, Canada.), Hooker, T.S, Tampe, P.A, Reid, D.M, Thorpe, T.A
Format: Article
Language:English
Subjects:
ANATOMIA DE LA PLANTA
ANATOMIE VEGETALE
Auxins
Botany
CRECIMIENTO
CROISSANCE
CULTIVO DE ORGANOS
CULTURE D'ORGANE
Density
Developmental biology
ETHYLENE PRODUCTION
GROWTH
GROWTH INHIBITORS
INHIBIDORES DEL CRECIMIENTO
INHIBITEUR DE CROISSANCE
LYCOPERSICON ESCULENTUM
ORGAN CULTURE
PELOS RADICALES
Physiology
PLANT ANATOMY
Plant growth
Plant roots
Plants
POIL ABSORBANT
PRODUCCION DE ETILENO
PRODUCTION D'ETHYLENE
RACINE
RAICES
Root growth
ROOT HAIRS
ROOT TIPS
ROOTS
Seedlings
Tomatoes
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Summary:The effect of jasmonic acid (JA) on growth, morphology, and development of roots and the possible interaction of JA with ethylene were investigated using isolated tomato roots (Lycopersicon esculentum Mill. cv Bush Beefsteak) cultured in vitro. JA had a number a direct effects on the growth and development of these isolated root tissues. Lower concentrations of JA (10-9-10-8M) in the culture medium promoted the frequency of lateral root initiation and lateral root elongation but had little effect on the elongation of the main axis. Higher (10-7M) concentrations of JA caused a rapid, irreversible inhibition of root growth, with lateral and main root axis elongation more severely affected than lateral root initiation. With 10-5M JA, the main root axis showed stunted growth, increased cellular vacuolation, and decreased cell elongation. Root apices were swollen and root hairs were aggregated closer to the root tip. In addition, phenolic compounds were present in the cytosol, and large cuboidal protein crystals were abundant in the epidermal and cortical cells of JA-treated roots. Treatment with ethylene mimicked some of the JA effects. Jasmonic acid-treated roots produced more ethylene than controls, but this was only evident after several days of culture. Inhibitors of ethylene action and synthesis did not block the effects of JA. We conclude that JA was not acting directly through ethylene.
ISSN:1058-5893
1537-5315
DOI:10.1086/297393