Super-Resolution Microscopy and Their Applications in Food Materials: Beyond the Resolution Limits of Fluorescence Microscopy

Super-resolution microscopy (SRM) techniques have revolutionized the process of obtaining images beyond Abbe’s diffraction limit (∼200 nm) of the light microscopes. It is possible to obtain images in 2D and 3D by reaching a lateral resolution of up to 10 nm of the cellular ultrastructure and biomole...

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Bibliographic Details
Published in:Food and bioprocess technology 2023-02, Vol.16 (2), p.268-288
Main Authors: Gallegos-Cerda, Susana Dianey, Hernández-Varela, Josué David, Chanona-Pérez, José Jorge, Arredondo Tamayo, Benjamín, Méndez Méndez, Juan Vicente
Format: Article
Language:English
Subjects:
Agriculture
Biomolecules
Biotechnology
Chemical compounds
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Confocal microscopy
Depletion
Fluorescence
Fluorescence microscopy
Fluorophores
Food
Food processing
Food Science
Image reconstruction
Localization
Microscopes
Microscopy
Nanotechnology
Review
Stimulated emission
Ultrastructure
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Summary:Super-resolution microscopy (SRM) techniques have revolutionized the process of obtaining images beyond Abbe’s diffraction limit (∼200 nm) of the light microscopes. It is possible to obtain images in 2D and 3D by reaching a lateral resolution of up to 10 nm of the cellular ultrastructure and biomolecules labelled with fluorophores. However, SRM is relatively unknown and rarely applied in food science, yet it has huge potential for the development of food nanotechnology. There is a focus on structured illumination microscopy (SIM), stimulated emission depletion (STED) microscopy, photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), and minimal photon fluxes (MINFLUX) microscopy as well as a compilation and discussion of the main fluorophores used in these techniques, the recent advances, microscope systems, challenges, and future perspectives of SRM in the food science. This review provides significant insights into SRM techniques, operation principles, recent advances, and applications in food materials. This contribution can be considered a brief guide for introducing non-experts in the food area to the knowledge of SRM techniques and contains useful information for experts in fluorescence and confocal microscopy interested in the use of SRM.
ISSN:1935-5130
1935-5149
DOI:10.1007/s11947-022-02883-4