Extraction, purification and characterization of phenazine from Pseudomonas aeruginosa isolate of wastewater sources: a panacea towards clinical pathogens

Today with the help of advancement in the biotechnological processes, researchers are concentrating more on economical and versatile ways to recover value-added products from natural resources such as waste waters. Pseudomonas aeruginosa is known to produce a broad range of secondary metabolites dur...

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Bibliographic Details
Published in:Applied nanoscience 2023-03, Vol.13 (3), p.2365-2378
Main Authors: Thacharodi, Aswin, Priyadharshini, R., Karthikeyan, G., Jeganathan, C., Reghu, Akhila. P., Pugazhendhi, Arivalagan
Format: Article
Language:English
Subjects:
Chemistry and Materials Science
Chromatography
Column chromatography
Fourier transforms
Fungicides
Gas chromatography
Mass spectrometry
Materials Science
Membrane Biology
Metabolites
Nanochemistry
Nanotechnology
Nanotechnology and Microengineering
Natural resources
NMR
Nuclear magnetic resonance
Original Article
Pathogens
Pseudomonas aeruginosa
Staphylococcus infections
Thin layer chromatography
Wastewater
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Summary:Today with the help of advancement in the biotechnological processes, researchers are concentrating more on economical and versatile ways to recover value-added products from natural resources such as waste waters. Pseudomonas aeruginosa is known to produce a broad range of secondary metabolites during its active growth phase. In this study, P aeruginosa was densely isolated from the waste dumping zone of the highly polluted Cooum River (CR), Tamil Nadu, India. The strain was identified through biochemical analysis and molecular characterization by 16S rRNA sequencing and named as JAAKPA. Enzyme profiling of the strain revealed that it had potential lipase and protease activities. Antibacterial and antifungal activities of the strain against ten clinical pathogens were tested. A significant antibacterial activity against Gram-positive strains such as methicillin-resistant Staphylococcus aureus (MRSA), Bacillus subtilis , and moderate antifungal activity against Candida albicans , were observed. The culture supernatant of JAAKPA was subjected to thin-layer chromatography and column chromatography to extract and purify the antimicrobial compound (Phenazine). The purified compound was further subjected to gas chromatography–mass spectrometry (GC-MS), fourier transform infrared spectroscopy (FTIR), and nuclear magnetic resonance (NMR) studies. The results revealed phenazine to be the major secondary metabolite responsible for the antimicrobial activities.
ISSN:2190-5509
2190-5517
DOI:10.1007/s13204-021-01944-y