Comparing the mesenchymal stem cells proliferation rate with different labeling assessments

Adipose tissue-derived mesenchymal stem cells (AD-MSCs) are an appropriate source for regenerative medicine and transplantation therapy due to their high proliferation rate in vitro. Bromodeoxyuridine (BrdU) marker is widely used for evaluating cell proliferation. BrdU cell labeling method has some...

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Bibliographic Details
Published in:Nucleus (Calcutta) 2023-04, Vol.66 (1), p.31-37
Main Authors: Ramezani, Maryam, Mirzaeian, Leila, Ghezelayagh, Zeinab, Ghezelayagh, Zahra, Ghorbanian, Mohammad Taghi
Format: Article
Language:English
Subjects:
Adipose tissue
Biomedical and Life Sciences
Body fat
Bromodeoxyuridine
Cell Biology
Cell culture
Cell growth
Cell proliferation
Cell self-renewal
Fetal calf serum
Growth rate
Kinases
Life Sciences
Mesenchymal stem cells
Original Article
Regenerative medicine
Stem cells
Transplantation
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Summary:Adipose tissue-derived mesenchymal stem cells (AD-MSCs) are an appropriate source for regenerative medicine and transplantation therapy due to their high proliferation rate in vitro. Bromodeoxyuridine (BrdU) marker is widely used for evaluating cell proliferation. BrdU cell labeling method has some limitations including its effects on cell proliferation and cellular mutations followed by an abnormality in the cell. On the other hand, the city of Kiel-67 (Ki-67) nuclear protein is expressed in dividing cells throughout the mitotic process. Our main objective was to compare the effects of BrdU and Ki-67 markers on self-renewal levels in AD-MSCs. In this study, the AD-MSCs were extracted from subcutaneous adipose tissue of adult rats and cultured in α-MEM supplemented with 10% fetal bovine serum (FBS). Fourth passage AD-MSCs after characterization were evaluated by immunocytochemical assay for BrdU and Ki-67 markers based on growth and proliferation rates of AD-MSCs at 24, 48, 72, and 96 h after culture. Our results show that the growth rate of AD-MSCs significantly changed at different times for both markers, although the growth and proliferation patterns were initially different in them. Unlike the BrdU, the Ki-67 endogenous marker had no side effect and interfered with cellular proliferation. It is opined that evaluating cell proliferation rate using the Ki-67 marker is more effective than the BrdU assay.
ISSN:0029-568X
0976-7975
DOI:10.1007/s13237-022-00415-1