Chrysin attenuates traumatic brain injury-induced recognition memory decline, and anxiety/depression-like behaviors in rats: Insights into underlying mechanisms

• Published in: Psychopharmacology 2020-06, Vol.237 (6), p.1607-1619
• Main Authors: Rashno, Masome, Ghaderi, Shahab, Nesari, Ali, Khorsandi, Layasadat, Farbood, Yaghoob, Sarkaki, Alireza
• Format: Article
• Language: English
• Subjects: Animal memory; Animals; Anti-inflammatory agents; Anxiety; Anxiety - drug therapy; Anxiety - etiology; Anxiety - psychology; Apoptosis; Apoptosis - drug effects; Apoptosis - physiology; Bcl-2 protein; Behavior; Biomedical and Life Sciences; Biomedicine; Brain; Brain - drug effects; Brain - physiology; Brain Injuries, Traumatic - complications; Brain Injuries, Traumatic - drug therapy; Brain Injuries, Traumatic - psychology; Cerebral cortex; Depression - drug therapy; Depression - etiology; Depression - psychology; Depression, Mental; Enzyme-linked immunosorbent assay; Flavonoids; Flavonoids - pharmacology; Flavonoids - therapeutic use; Hippocampus; Immunohistochemistry; Inflammation; Injuries; Male; Memory; Memory Disorders - drug therapy; Memory Disorders - etiology; Memory Disorders - metabolism; Memory Disorders - psychology; Mental depression; Neurosciences; Original Investigation; Pharmacology/Toxicology; Psychiatry; Rats; Rats, Wistar; Recognition, Psychology - drug effects; Risk factors; Traumatic brain injury
• ISSN: 0033-3158; 1432-2072
• DOI: 10.1007/s00213-020-05482-3

Rationale Cortical and hippocampal neuronal apoptosis and neuroinflammation are associated with behavioral deficits following traumatic brain injury (TBI). Objectives The present study was designed to investigate the potential protective effects of flavonoid chrysin against TBI-induced vestibulomotor impairment, exploratory/locomotor dysfunctions, recognition memory decline, and anxiety/depression-like behaviors, as well as the verified possible involved mechanisms. Methods Chrysin (25, 50, or 100 mg/kg/day; P.O.) was administered to rats immediately after diffuse TBI induction, and it was continued for 3 or 14 days. Behavioral functions were assessed by employing standard behavioral paradigms at scheduled points in time. Three days post-TBI, inflammation status was assayed in both cerebral cortex and hippocampus using ELISA kits. Moreover, apoptosis and expression of Bcl-2 family proteins were examined by TUNEL staining and immunohistochemistry, respectively. Results The results indicated that treatment with chrysin improved vestibulomotor dysfunction, ameliorated recognition memory deficit, and attenuated anxiety/depression-like behaviors in the rats with TBI. Chrysin treatment also modulated inflammation status, reduced apoptotic index, and regulated Bcl-2 family proteins expression in the brains of rats with TBI. Conclusions In conclusion, the results suggest that chrysin could be beneficial for protection against TBI-associated behavioral deficits, owing to its anti-apoptotic and anti-inflammatory properties.