Use of Magnetic Carbon Nanocomposites in the Formation of a Recognition Layer of a Piezoelectric Immunosensor for the Determination of Penicillin G

• Published in: Journal of analytical chemistry (New York, N.Y.) N.Y.), 2023-04, Vol.78 (4), p.488-496
• Main Authors: Bizina, E. V., Farafonova, O. V., Zolotareva, N. I., Grazhulene, S. S., Ermolaeva, T. N.
• Format: Article
• Language: English
• Subjects: Analysis; Analytical Chemistry; Carbon; Carbon compounds; Carbon nanotubes; Chemistry; Chemistry and Materials Science; Composite materials; Immunosensors; Infrared spectroscopy; Iron oxides; Magnetic properties; Minimum weight; Nanocomposites; Nanoparticles; Penicillin; Penicillin G; Piezoelectric devices; Piezoelectricity; Recognition; Sensors
• ISSN: 1061-9348; 1608-3199
• DOI: 10.1134/S1061934823040068

Conditions for the formation of a recognition layer of a piezoelectric immunosensor based on magnetic carbon nanocomposites (MCNCs) under the action of an external magnetic field are studied. The effects of the size and number of magnetic nanoparticles (MNPs) in the composite on the analytical characteristics of the gravimetric immunosensor are revealed. Scanning electron microscopy is used to determine the average sizes of Fe 3 O 4 magnetic nanoparticles synthesized by coprecipitation. It is noted that the minimum weight and stability of the recognition layer were observed for the nanocomposite obtained at a ratio of carbon nanotubes and MNPs with an average diameter of 22 nm equal to 3 : 1. The formation of peptide bonds between the MCNCs and a penicillin G conjugate was established by IR spectrometry. It was shown that the use of magnetic carbon nanocomposites in the formation of a recognition layer makes it possible to significantly simplify the procedure for preparing a piezoelectric sensor for analysis and reduce its duration from 24 to 1.5 h. The range of the determined antibiotic concentrations is 1–450 ng/mL, the limit of detection is 0.5 ng/mL.