Dynamic and structural properties of porcine serum albumins

Porcine serum albumin (PSA) is one of the promising biomarkers for pork detection. Pork contamination is a serious concern for the global halal food industry since many manufacturers mix pork into halal beef products to reduce production costs. Many studies have thus been devoted to designing effect...

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Bibliographic Details
Published in:Molecular simulation 2023-06, Vol.49 (9), p.877-884
Main Authors: Niramitranon, Jitti, Japrung, Deanpen, Boonmee, Apaporn, Koonawootrittriron, Skorn, Suwanasopee, Thanathip, Jattawa, Danai, Pongprayoon, Prapasiri
Format: Article
Language:English
Subjects:
albumin
Biomarkers
Biosensors
Cattle
Contaminants
Electropositivity
Halal food
Homology
Hydrophobicity
MD simulations
Molecular dynamics
Porcine serum albumin
Pork
Production costs
Serum albumin
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Summary:Porcine serum albumin (PSA) is one of the promising biomarkers for pork detection. Pork contamination is a serious concern for the global halal food industry since many manufacturers mix pork into halal beef products to reduce production costs. Many studies have thus been devoted to designing effective PSA-detecting biosensors. PSA is closely related to Bovine serum albumin (BSA); therefore; the molecular insight into PSA characteristics becomes crucial to identify PSA. To understand PSA properties, Molecular dynamic (MD) simulations were employed. The three-dimensional structures of PSA were obtained from homology modelling and Alphafold. Both models give similar results. PSA seems to have high hydrophobicity and unique electrostatic properties. Unlike BSA, PSA has no large electropositive patch on the rear of domain III. This property can be used to differentiate PSA from BSA. In the case of drug sites, PSA provides comparable sizes of drug sites to those of canine serum albumin (CSA) which are larger than those of bovine, human and feline albumins. Such larger binding pockets can imply the ability of PSA to accommodate a broader spectrum of ligands. The findings here, especially the difference between BSA and PSA, can serve as a base to design effective biosensors to detect PSA contaminants.
ISSN:0892-7022
1029-0435
DOI:10.1080/08927022.2023.2200485