Effects of dietary Plantago ovata seed extract administration on growth performance and immune function of common carp (Cyprinus carpio) fingerling exposed to ammonia toxicity

• Published in: Veterinary research communications 2023-06, Vol.47 (2), p.731-744
• Main Authors: Ahmadifar, Ehsan, Kalhor, Naser, Yousefi, Morteza, Adineh, Hossein, Moghadam, Mohsen Shahriari, Sheikhzadeh, Najmeh, Moonmanee, Tossapol, Hoseinifar, Seyed Hossein, Van Doan, Hien
• Format: Article
• Language: English
• Subjects: Ammonia; Ammonia - toxicity; Animal Feed - analysis; Animals; Antioxidants; Aquatic animals; Biomedical and Life Sciences; Carp; Carps; CYP1A protein; Cyprinus carpio; Cytochrome P450; Diet; Diet - veterinary; Dietary Supplements; Food conversion; Gene expression; Genes; Hsp70 protein; Immune response; Immunity; Interleukin 10; Life Sciences; Lysozyme; Medicinal plants; Osmoregulation; Oxidative stress; Plant Extracts - pharmacology; Plantago - metabolism; Plantago ovata; Toxicity; Veterinary Medicine/Veterinary Science; Well being; Zoology; γ-Interferon
• ISSN: 0165-7380; 1573-7446
• DOI: 10.1007/s11259-022-10034-5

Medicinal plants are powerful antioxidants which can improve well-being and suppress oxidative stress caused by environmental toxins in aquatic animals. In this regard, the present research was designed to show the potential effects of psyllium ( Plantago ovata ) seed extract (PSE) on the growth, and immune responses of common carp Cyprinus carpio exposed to acute ammonia toxicity. To perform the study, fish were fed with diets containing 0 (T0), 0.25 (T1), 0.5 (T2), and 1% (T3) PSE for 60 days, and then exposed to ammonia (0.5 mg L −1 ) for 3 h. The findings showed that fish given the T1 diet outperformed the T3 and control groups in terms of ultimate weight, weight increase, and food conversion ratio. Additionally, the T1 group showed a significantly higher level of total protein and serum lysozyme activity than the other treatment groups. Moreover, the highest serum total immunoglobulin values were recorded in T1 and T2 groups. The results showed that PSE, especially at moderate levels, could successfully upregulate the transcription of immune-related genes ( IFN-γ , Hsp70, TNF-ɑ , IL-1ß , IL-10 , and IgE ) compared to the control group after exposure to ammonia. Furthermore, improving ammonia-induced down regulations of antioxidant-related gene expressions ( CYP1A, SOD , and GPX ) was observed in fish fed with PSE-included diets compared to the control one. However, PSE-supplemented diets did not affect the mRNA expression level of  CAT . Regarding tight junction-associated genes, the higher mRNA expression level of occludin was observed in the T1 group, whereas the downregulation of CLD3 gene occurred in all experimental groups. Conversely, significant upregulation of osmoregulation-associated gene ( NKA ) was recorded in all experimental groups compared to the control one. Therefore, the administration of PSE (0.25% of the diet) for 60 days is recommended to increase growth performance, improve health, and increase the resistance of common carp to oxidative stress caused by ammonia. Graphical abstract