Effect of post-harvest processing methods on the microbial safety of edible seaweed

• Published in: Journal of applied phycology 2023-06, Vol.35 (3), p.1331-1346
• Main Authors: Vorse, Jessica G., Moody, Colleen T., Massoia, Lyle C., Perry, Jennifer J., Burkholder, Kristin M., Byron, Carrie J.
• Format: Article
• Language: English
• Subjects: Air; Algae; Ascophyllum nodosum; Bacteria; Biomedical and Life Sciences; E coli; Ecology; Escherichia coli; Foodborne pathogens; Freeze drying; Freshwater & Marine Ecology; Harvesting; Inoculation; Kelp; Life Sciences; Listeria monocytogenes; Methods; Microorganisms; Pathogens; Plant Physiology; Plant Sciences; Replication; Saccharides; Saccharina latissima; Safety; Salmonella enterica; Seaweed industry; Seaweed products; Seaweeds; Staphylococcus aureus; Storage temperature; Vibrio parahaemolyticus; Vibrio vulnificus
• ISSN: 0921-8971; 1573-5176
• DOI: 10.1007/s10811-023-02937-w

The American seaweed industry is growing, primarily into the edible sector, and more seaweed products are available for human consumption. It is necessary to evaluate the safety of industry’s current post-harvest storage and processing methods to ensure the risk of foodborne pathogens on edible seaweed remains low. We evaluated the pathogen load of edible kelp post-harvest under three different storage temperatures (4˚C, 10˚C, 20˚C) and two different drying methods (air- and freeze-drying). The focal pathogens for this research included: Listeria monocytogenes, Salmonella enterica, Staphylococcus aureus, pathogenic Escherichia coli, Vibrio vulnificus and Vibrio parahaemolyticus . We tested all six pathogens under each treatment condition on both sugar kelp ( Saccharina latissima) and rockweed ( Ascophyllum nodosum ) as these are the most commonly farmed and wild-harvested species in Maine, respectively. We inoculated a known concentration of pathogen onto freshly harvested kelp, treated it under a storage temperature or drying method, and sampled it over time to determine the impact of treatment on pathogen load. Our results showed that storage at 20˚C can lead to replication while storage at 4˚C and 10˚C halted the replication of focal pathogens. Both air-drying and freeze-drying produced significant log scale reductions in surface pathogen load for all focal pathogens. Additionally, air-drying reduced pathogen load more than freeze-drying for a majority of pathogens and storing dried kelp for 6-weeks further reduced pathogen load across all cases. These results are promising for industry as they corroborate historical evidence that current post-harvest storage and processing conditions are producing products safe for human consumption.